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|標題:||Analysis of the biosynthesis genes and chemical components of the capsule of Avibacterium paragallinarum||作者:||Wu, J.R.
|關鍵字:||Avibacterium paragallinarum;Capsule biosynthesis;Capsular;polysaccharide;pasteurella-multocida;haemophilus-paragallinarum;molecular-cloning;escherichia-coli;hemophilus-paragallinarum;chondroitin synthase;hyaluronan synthase;identification;polysaccharide;influenzae||Project:||Veterinary Microbiology||期刊/報告no：:||Veterinary Microbiology, Volume 145, Issue 1-2, Page(s) 90-99.||摘要:||
The aim of this study was to investigate biosynthesis genes and chemical components of the capsule of Avibacterium paragallinarum. The sequence of a 10-kb region containing the capsule biosynthetic locus of Av. paragallinarum was determined. Two reference strains, i.e., 221 (serovar A) and H18 (serovar C), together with four Taiwanese field strains (all serovar C) were sequenced. The results showed that there are two genotypes (I and II) of the capsule biosynthetic locus in Av. paragallinarum, and the capsule genotype is independent of the serovar. The capsule biosynthetic loci of genotypes I and II consisted of six and five genes, respectively. The genotype I genes encoded proteins that are most similar to proteins from Pasteurella multocida capsule types A and F while the genotype II genes encoded proteins most similar to proteins from P. multocida capsule type D and Escherichia coli K5. The results suggested that genotype I strains contain hyaluronan or chondroitin in the capsule wall while genotype II contain heparosan. Enzymatic digestion of the capsule materials extracted from Av. paragallinarum showed that genotype I strains contained chondroitin while genotype II strains contained heparosan in the capsule. This is the first report on the existence of different genotypes of capsule biosynthesis genes in Av. paragallinarum and the presence of chondroitin and heparosan as chemical components of the capsule of Av. paragallinarum. (c) 2010 Elsevier B.V. All rights reserved.
|Appears in Collections:||微生物暨公共衛生學研究所|
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