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標題: Functional expression of the recombinant ATPase of orf virus
作者: Lin, F.Y.
Chan, K.W.
Wang, H.C.
Hsu, W.L.
Wong, M.L.
關鍵字: vaccinia virus;outbreak;protein;parapoxviruses;product;ntpase;goats
Project: Archives of Virology
期刊/報告no:: Archives of Virology, Volume 155, Issue 10, Page(s) 1701-1705.
Nucleotide sequence analysis has indicated that the A32L gene of orf virus can encode an ATPase (Chan et al. in Gene 432:44-53, 2009). In this work, we cloned the A32L gene into a prokaryotic expression vector, and the recombinant protein was expressed and purified. The antigenicity of recombinant ATPase was examined by immunoblotting, and its identity was confirmed by mass spectrometry. The ATP hydrolysis function of the purified recombinant protein was examined, and our results showed that it exhibited the ATPase activity. Similar to other viral ATPases, the ATPase of orf virus remained active in the presence of different divalent ions; nevertheless, unlike other viral ATPases, our recombinant ATPase exhibited similar enzymatic activity in reaction buffers of different pH.
ISSN: 0304-8608
DOI: 10.1007/s00705-010-0754-5
Appears in Collections:微生物暨公共衛生學研究所

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