Please use this identifier to cite or link to this item:
標題: Phylogenetic analysis of parapoxviruses and the C-terminal heterogeneity of viral ATPase proteins
作者: Chan, K.W.
Yang, C.H.
Lin, J.W.
Wang, H.C.
Lin, F.Y.
Kuo, S.T.
Wong, M.L.
Hsu, W.L.
關鍵字: Parapoxviruses;Orf virus;A32L gene;B2L gene;E3L gene;orf-virus;vaccinia virus;interferon resistance;sequence alignment;gene;outbreak;goats;identification;integrins;homolog
Project: Gene
期刊/報告no:: Gene, Volume 432, Issue 1-2, Page(s) 44-53.
Two outbreaks of orf virus (a parapoxvirus) infection in goats found in Nantou and Taiping of central Taiwan were investigated. The nucleotide and the amino acid sequences of viral B2L, E3L and A32L genes in these two outbreaks were analyzed, and each of their phylogenetic trees were also constructed. In the A32L gene, an unexpected deletion of 24 nucleotides was found in the Taiping strain. The A32L gene can encode an ATPase and is supposed to be involved in virion DNA packaging. The 24 nucleotides correspond to 8 amino acids residues of the viral ATPase, which are located near the C-terminal region of the enzyme. Moreover, two copies of the RGD sequence at C-terminal region of ATPase were found in the Nantou strain. The 24 nucleotide difference in the A32L gene indicated that the Nantou strain and the Taiping strain were two separate strains, and it can be used in differential molecular diagnosis. Moreover, the C-terminal heterogeneity was found to be a general feature of the viral ATPase. Lastly, similar functional motifs of the ATPase and the Ras proto-oncoprotein (a GTPase) are discussed. (c) 2008 Elsevier B.V. All rights reserved.
ISSN: 0378-1119
DOI: 10.1016/j.gene.2008.10.029
Appears in Collections:微生物暨公共衛生學研究所

Show full item record

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.