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標題: Differentially expressed transcripts in shell glands from low and high egg production strains of chickens using cDNA microarrays
作者: Yang, K.T.
Lin, C.Y.
Liou, J.S.
Fan, Y.H.
Chiou, S.H.
Huang, C.W.
Wu, C.P.
Lin, E.C.
Chen, C.F.
Lee, Y.P.
Lee, W.C.
Ding, S.T.
Cheng, W.T.K.
Huang, M.C.
關鍵字: chickens;shell glands;egg production;cDNA microarrays;RT-PCR;eggshell matrix protein;mx-protein;antiviral activity;dna;microarrays;sequence tags;gene;identification;ovocalyxin-32;generation;resources
Project: Animal Reproduction Science
期刊/報告no:: Animal Reproduction Science, Volume 101, Issue 1-2, Page(s) 113-124.
We have constructed a tissue-specific in-house cDNA microarray to identify differentially expressed transcripts in shell glands from low (B) and high (1-2) egg production strains of Taiwanese country chickens during their egg-laying period. The shell gland cDNA library was constructed from the high egg production strain. cDNA clones (7680) were randomly selected and their 5'-end sequences characterized. After excluding overlapping sequences, an in-house cDNA microarray, representing 2743 non-redundant transcripts, was generated for functional genomic studies. Using our microarray, we have successfully identified 85 differentially expressed transcripts from the two different strains of chicken shell glands. In this study, 34 of these transcripts were associated with signal transduction, protein biosynthesis, cell adhesion, cellular metabolism, skeletal development, cell organization and biogenesis. We selected a number of the differentially expressed transcripts for further validation using semi-quantitative RT-PCR. These included elongation factor 2 (EEF2), ovocalyxin-32 (OCX-32) and annexin A2 (ANXA2) which were expressed at high levels in the chicken shell glands of the B strain and, in contrast, the coactosin-like protein (COTL1), transcription factor SOX18 and MX protein were more highly expressed in the L2 strain. Our results suggest that these differentially expressed transcripts may be suitable to use as molecular markers for high rates of egg production, and now need to be investigated further to assess whether they can be applied for use in breeding selection programs in Taiwanese country chickens. (C) 2006 Elsevier B.V. All rights reserved.
ISSN: 0378-4320
DOI: 10.1016/j.anireprosci.2006.09.004
Appears in Collections:微生物暨公共衛生學研究所

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