Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66631
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dc.contributor.authorWu, J.R.en_US
dc.contributor.author張伯俊zh_TW
dc.contributor.authorShien, J.H.en_US
dc.contributor.authorShieh, H.K.en_US
dc.contributor.authorHu, C.C.en_US
dc.contributor.authorGong, S.R.en_US
dc.contributor.authorChen, L.Y.en_US
dc.contributor.authorChang, P.C.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-11T05:46:51Z-
dc.date.available2014-06-11T05:46:51Z-
dc.identifier.issn0378-1097zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/66631-
dc.description.abstractWe have identified a new phoX gene encoding the monomeric alkaline phosphatase from Pasteurella multocida X-73. This gene was not found in the published genome sequence of Pasteurella multocida pm70. Characterization of the recombinant PhoX of Pasteurella multocida X-73 showed that it is a monomeric enzyme, activated by Ca2+ and possibly secreted by the Tat pathway. These features distinguish phosphatases of the PhoX family from those of the PhoA family. All proteins of the PhoX family were found to contain a conserved motif that shares significant sequence homology with the calcium-binding site of a phosphotriesterase known as diisopropylfluorophosphatase. Site-directed mutagenesis revealed that D527 of PhoX might be the ligand bound to the catalytic calcium. This is the first report on identification of homologous sequences between PhoX and the phosphotriesterase and on the potential calcium-binding site of PhoX.en_US
dc.language.isoen_USzh_TW
dc.relationFems Microbiology Lettersen_US
dc.relation.ispartofseriesFems Microbiology Letters, Volume 267, Issue 1, Page(s) 113-120.en_US
dc.relation.urihttp://dx.doi.org/10.1111/j.1574-6968.2006.00542.xen_US
dc.subjectalkaline phosphataseen_US
dc.subjectPhoXen_US
dc.subjectPasteurella multocidaen_US
dc.subjectescherichia-colien_US
dc.subjectvibrio-choleraeen_US
dc.subjectgluconolactonaseen_US
dc.subjectidentificationen_US
dc.subjectmembraneen_US
dc.subjectsequenceen_US
dc.subjectassayen_US
dc.titleCloning of the gene and characterization of the enzymatic properties of the monomeric alkaline phosphatase (PhoX) from Pasteurella multocida strain X-73en_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1111/j.1574-6968.2006.00542.xzh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:微生物暨公共衛生學研究所
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