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標題: Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA
作者: Hsu, Y.M.
Shieh, H.K.
Chen, W.H.
Shiang, J.H.
Chang, P.C.
關鍵字: Avibacterium paragallinarum;subunit vaccine;haemagglutination;HagA;nontypable haemophilus-influenzae;outer-membrane protein;hemophilus-paragallinarum;infectious coryza;actinobacillus-actinomycetemcomitans;serological classification;immunological properties;molecular analysis;serotype-a;psi-blast
Project: Veterinary Microbiology
期刊/報告no:: Veterinary Microbiology, Volume 122, Issue 3-4, Page(s) 280-289.
Inactivated vaccines of Avibacterium paragallinarum provide protection and reduce the economic losses caused by infectious coryza. However, inactivated bacterins provide protection only against the Page serovars included in the vaccine. In this study, we investigated the immunological properties of a functional recombinant haemagglutinin protein (rHagA) derived from a Taiwan isolate strain A9 as the immunogen for vaccination. The rHagA subunit vaccine protected 71% of immunized chickens against 10(10) colony-forming units (CFU) of viable A9. Vaccinated chickens which showed no clinical signs of coryza developed haernagglutination inhibition (HI) titers of 1: 10 or greater. Haemagglutination (HA) of serovars A and C was not affected by the presence of rHagA specific antiserum. The HA of rHagA could only be induced against formaldehyde-fixed chicken red blood cells (FA-RBCs). These results suggested that HagA is a moderate immunogen and might not be a major haemagglutinin in vivo. However, HagA might be involved in haemagglutination when treated serovar C aggregates fixed RBCs in vitro. (c) 2007 Elsevier B.V. All rights reserved.
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2007.01.014
Appears in Collections:微生物暨公共衛生學研究所

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