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標題: Yeast-expressed classical swine fever virus glycoprotein E2 induces a protective immune response
作者: Lin, G.J.
Liu, T.Y.
Tseng, Y.Y.
Chen, Z.W.
You, C.C.
Hsuan, S.L.
Chien, M.S.
Huang, C.J.
關鍵字: Classical swine fever virus (CSFV);Pichia pastoris expression system;Recombinant E2 protein;Subunit marker vaccine;subunit marker vaccine;hog-cholera virus;pichia-pastoris;virulent;antibody;protein;single;strain
Project: Veterinary Microbiology
期刊/報告no:: Veterinary Microbiology, Volume 139, Issue 3-4, Page(s) 369-374.
Classical swine fever (CSF) is an economically important swine disease worldwide. The glycoprotein E2 of classical swine fever virus (CSFV) is a viral antigen that can induce a protective immune response against CSF. A recombinant E2 protein was constructed using the yeast Pichia pastoris expression system and evaluated for its vaccine efficacy. The yeast-expressed E2 (yE2) was shown to have N-linked glycosylation and to form homodimer molecules. Four 6-week-old specified-pathogen-free (SPF) piglets were intramuscularly immunized with yE2 twice at 3-week intervals. All yE2-vaccinated pigs could mount an anamnestic response after booster vaccination with neutralizing antibody titers ranging from 1:96 to 1:768. Neutralizing antibody titers at 10 weeks post booster vaccination ranged from 1:16 to 1:64. At this time, the pigs were subjected to challenge infection with a dose of 1 x 10(5) TCID(50) (50% tissue culture infective dose) virulent CSFV strain. At 1 week post challenge infection, all of the yE2-immunized pigs were alive and without symptoms or signs of CSF. Neutralizing antibody titers at this time ranged from 1:4,800 to 1:12,800 and even to 1:51,200 one week later. In contrast, the control pigs continuously exhibited signs of CSF and had to be euthanized because of severe clinical symptoms at 6 days post challenge infection. All of the yE2-vaccinated pigs were E(rns) antibody negative and had seroconverted against E(rns) by post challenge day 11, suggesting that yE2 is a potential DIVA (differentiating infected from vaccinated animals) vaccine. The yeast-expressed E2 protein retains correct immunogenicity and is able to induce a protective immune response against CSFV infection. (C) 2009 Elsevier B.V. All rights reserved.
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2009.06.027
Appears in Collections:微生物暨公共衛生學研究所

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