Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66668
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dc.contributor.authorHuang, C.J.en_US
dc.contributor.author黃千衿zh_TW
dc.contributor.authorChien, M.S.en_US
dc.contributor.authorHu, C.M.en_US
dc.contributor.authorChen, C.W.en_US
dc.contributor.authorHsieh, P.C.en_US
dc.contributor.author簡茂盛zh_TW
dc.date2006zh_TW
dc.date.accessioned2014-06-11T05:46:57Z-
dc.date.available2014-06-11T05:46:57Z-
dc.identifier.issn0166-0934zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/66668-
dc.description.abstractE-rns is an envelope glycoprotein of classical swine fever virus (CSFV) with RNase activity. The purpose of this study was to produce an active E-rns for further applications using the yeast secreted expression system. The E-rns gene was cloned into the expression vector pGAPZ alpha C which was introduced into Pichia pastoris. Expression of Erns protein in culture supernatant was confirmed by Western blot analysis using both the monoclonal antibody against CSFV E-rns and CSFV-positive swine serum. The yeast-expressed E-rns (yE(rns)) was shown to have N-linked glycosylation and to form homodimer of 74 kDa molecules. All monomer, homodimer, and deglycosylated forms of yE(rns) demonstrated intrinsic ribonuclease activity and a clear preference for uridine-rich sequence. A direct sandwich blocking enzyme-linked immunosorbent assay (ELISA) based on the yE(rns) was developed with a high sensitivity and specificity. The yE(rns) which possesses enzymatic activity and retains antigenicity may provide a useful material for developing a diagnostic kit. (c) 2005 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Virological Methodsen_US
dc.relation.ispartofseriesJournal of Virological Methods, Volume 132, Issue 1-2, Page(s) 40-47.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.jviromet.2005.08.020en_US
dc.subjectclassical swine fever virusen_US
dc.subjectglycoproteinen_US
dc.subjectPichia pastorisen_US
dc.subjectRNaseen_US
dc.subjectblocking ELISAen_US
dc.subjectprotein e-rnsen_US
dc.subjectpichia-pastorisen_US
dc.subjectmethylotrophic yeasten_US
dc.subjectpestivirusen_US
dc.subjectidentificationen_US
dc.subjectribonucleaseen_US
dc.subjectdiagnosisen_US
dc.subjectimmunityen_US
dc.subjectvaccineen_US
dc.subjectcellsen_US
dc.titleSecreted expression of the classical swine fever virus glycoprotein E-rns in yeast and application to a sandwich blocking ELISAen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.jviromet.2005.08.020zh_TW
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
Appears in Collections:微生物暨公共衛生學研究所
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