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標題: Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses
作者: Huang, C.J.
Hung, J.J.
Wu, C.Y.
Chien, M.S.
關鍵字: multiplex PCR;pseudorabies virus;porcine parvovirus;porcine;circovirus;polymerase-chain-reaction;experimental reproduction;wasting disease;pigs;coinfection;dna
Project: Veterinary Microbiology
期刊/報告no:: Veterinary Microbiology, Volume 101, Issue 3, Page(s) 209-214.
A multiplex PCR (mPCR) assay was developed and subsequently evaluated for its effectiveness as a means to simultaneously detect multiple viral infections of swine. Specific primers for each of four common DNA viruses, namely, pseudorabies virus (PRV), porcine circovirus type I (PCV1), porcine circovirus type 11 (PCV2), and porcine parvovirus (PPV), were used for testing procedure. The assay was shown to be highly sensitive in that as little as 10(-4) ng of each of the respective amplicons (approximately equal to 10,000 molecules) was detected when a composite of all four viruses (including both field and gene-deleted permutations of PRV) was tested as a single sample. It was also effective for detecting one or more of these same viruses in various combinations in specimens including lymph nodes, lungs, spleens, and tonsils collected from clinically ill pigs, and in specimens in spleen collected from aborted fetuses. The relative efficiency (compared to performing separate assays for each virus) and apparent sensitivity of mPCR suggest its potential application for routine molecular diagnostic purposes. (C) 2004 Elsevier B.V. All rights reserved.
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2004.04.007
Appears in Collections:微生物暨公共衛生學研究所

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