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標題: Increased survival in dogs with malignant mammary tumours overexpressing HER-2 protein and detection of a silent single nucleotide polymorphism in the canine HER-2 gene
作者: Hsu, W.L.
Huang, H.M.
Liao, J.W.
Bong, M.L.
Chang, S.C.
關鍵字: HER-2;Malignant mammary tumour;Canine;Prognosis;Survival;in-situ hybridization;human-breast-cancer;oncogene;carcinomas;amplification;prognosis;c-erbb-2
Project: Veterinary Journal
期刊/報告no:: Veterinary Journal, Volume 180, Issue 1, Page(s) 116-123.
The aims of this study were to explore the relationship between clinical outcome and HER-2 protein expression in dogs with malignant mammary tumours after surgery and to determine the sequence of the HER-2 gene. Tissues from 91 dogs diagnosed with malignant mammary tumours, six with benign lesions and two normal mammary glands were analysed for the expression of HER-2 by immunohistochemistry (IHC). Exons 14-18 of the HER-2 gene were amplified by reverse transcriptase polymerase chain reaction and sequenced in 19 tumours with IHC scores >2+; larger coding regions of HER-2 were analysed in live tumours. HER-2 protein overexpression was found in 27/91 (29.7%) of canine malignant mammary tumours. Dogs With HER-2-overexpressing malignant mammary tumours tended to have a higher survival rate than those that expressed it normal level of HER-2 within 2 years of surgery. In 3/19 cases, HER-2 sequences had a single nucleotide polymorphism, T(1725)C. with no change in the amino acid identity (Cys). No other sequence aberration of HER-2 was found. (c) 2007 Elsevier Ltd. All rights reserved.
ISSN: 1090-0233
DOI: 10.1016/j.tvjl.2007.10.013
Appears in Collections:微生物暨公共衛生學研究所

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