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標題: Identification, expression and antigenic analysis of recombinant hemagglutinin proteins of canine distemper virus
作者: Chan, K.W.
Hsieh, H.H.
Wang, H.C.
Lee, Y.J.
Sung, M.H.
Wong, M.L.
Hsu, W.L.
關鍵字: Canine distemper virus;Hemagglutinin;ELISA;naturally infected-dogs;measles;residues;fusion
Project: Journal of Virological Methods
期刊/報告no:: Journal of Virological Methods, Volume 155, Issue 1, Page(s) 18-24.
Canine distemper (CD) is a widely distributed disease of dogs, caused by the canine distemper virus (CDV). In the present study, the gene encoding the hemagglutinin (H) protein of a CDV isolate from central Taiwan was sequenced and compared with other strains. Sequence variations were noticed in the H gene from the field CDV strain that had previously been implicated in the increasing incidence of CD. To establish a serology-based diagnostic test, the full-length H protein, as well as five deletion mutants of a recombinant H protein of the local isolate, were produced using an E. coli expression system. Three truncated recombinant proteins with relatively high expression levels, designated HM3, HM4 and HIV15, were used as antigens to examine their reactivity with canine sera. By using three negative sera and 17 CD-positive sera, the high specificity of recombinant H proteins was observed by ELISA. In addition, immunoblotting demonstrated that all three purified recombinant proteins exhibit an antigenic property recognized by the serum of a CD-suspected dog. (c) 2008 Elsevier B.V. All rights reserved.
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2008.09.024
Appears in Collections:微生物暨公共衛生學研究所

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