Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66684
DC FieldValueLanguage
dc.contributor.authorWong, M.L.en_US
dc.contributor.author張天傑zh_TW
dc.contributor.authorLiu, J.J.en_US
dc.contributor.authorHuang, C.J.en_US
dc.contributor.authorChen, J.W.en_US
dc.contributor.authorChang, T.J.en_US
dc.contributor.author黃千衿zh_TW
dc.date1998zh_TW
dc.date.accessioned2014-06-11T05:46:59Z-
dc.date.available2014-06-11T05:46:59Z-
dc.identifier.issn0920-8569zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/66684-
dc.description.abstractA cDNA of the 3'-terminus of classical swine fever virus (LPC vaccine strain) was cloned and sequenced. The 3431 nucleotides and deduced amino acid sequences were compared with those of other pestiviruses, and the similarity of nucleotide sequences and deduced amino acid sequences were found to be 84-95% and 95-98%, respectively. Similar to other isolates of classical swine fever virus, the sequenced region included the non-structural gene p58 (NS5A) and part of p76 (NS5B) gene. The p76 gene of LPC vaccine strain also contained a highly conserved motif G-D-D (Gly-Asp-Asp) that is present in the RNA replicase of positive-stranded RNA viruses. With the sequence data currently available, we carried out a phylogenetic analysis and obtained a genealogical relationship among members of the classical swine fever virus. The sequence has been submitted to GeneBank with an accession number AF001986.en_US
dc.language.isoen_USzh_TW
dc.relationVirus Genesen_US
dc.relation.ispartofseriesVirus Genes, Volume 17, Issue 3, Page(s) 213-218.en_US
dc.relation.urihttp://dx.doi.org/10.1023/a:1008061603194en_US
dc.subjectpestivirusen_US
dc.subjectclassical swine fever virusen_US
dc.subjectRT-PCRen_US
dc.subjectsequencingen_US
dc.subjecthog-cholera virusen_US
dc.subjectviral diarrhea virusen_US
dc.subjectrna virusesen_US
dc.subjectpestivirusen_US
dc.subjectcdnaen_US
dc.subjectidentificationen_US
dc.subjectglycoproteinsen_US
dc.titleMolecular cloning and nucleotide sequence of 3 '-terminal region of classical swine fever virus LPC vaccine strainen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1023/a:1008061603194zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
Appears in Collections:微生物暨公共衛生學研究所
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