Please use this identifier to cite or link to this item:
DC FieldValueLanguage
dc.description.abstractThe diarrheagenic disease Salmonellosis caused by Salmonella choleraesuis and S. typhimurium is among the most important bacterial diseases worldwide and both pathogen can induce gastroenteritis, interstitial pneumonia and systemic septicemia in swine. After infection, pathogenic Salmonella serovars will encounter the intracellular killing by host phagocytic cells during internalization, but Salmonella has been proved to be able to survive and even replicate in phagocytic cells to cause systemic infection. Although Salmonella is thought to be an opportunistic pathogen in pig farm, it will induce serious economic problem after outbreak and hard to eradicate from a contaminated farm. According to the survey of Animal disease diagnostic center, 70% from the submitted swine cases were identified to be affected or associated with Salmonella co-infection. Those Salmonella isolates also showed multi-drugs resistance from the affected clinical cases. Because Salmonella invasion always start from the mucosal surfaces of gastrointestinal tract and microorganisms will transit across the mucosa during the early steps of the infections. Therefore, to elicit the local immune response to block the virulence determinants of Salmonella on mucus is extremely important to prevent both infection and the following disease development. However, most of these virulence factors are poorly immunogenic by mucosal administered route. Recently, a number of strategies have been developed to overcome this problem, including suitable mucosal adjuvant and vector vaccine delivers used. One of the approaches that can be pursued to elicit efficient mucosal immunity is the use of attenuated strains to deliver heterologous antigens by the mucosal route. Recent studies indicate that the attenuated Salmonella is a good candidate for immunization and also could be utilized for a potential vaccine vector to deliver multivalent heterologous antigens at mucosal administered routes. But the antibiotic gene inserted into the attenuated Salmonella Choleraesuis genome may raise a public health concern. Therefore, the purpose of this project is to develope an antibiotic gene-free Salmonella choleraesuis knockout strain and an over attenuated phoQ mutant. And we wish to develope a safe and immuno-protective live attenuated Salmonella vaccine in the near furture.en_US
dc.subjectAttenuated Vaccineen_US
dc.subject畜牧獸醫類, 生物技術zh_TW
dc.subjectSalmonella Choleraesuisen_US
dc.subjectAntibiotic Gene Freeen_US
dc.title豬Salmonella choleraesuis無抗藥標誌基因缺損活菌減毒疫苗之開發zh_TW
dc.titleDevelopment of an Antibiotic-Gene Free Live Attenuated Vaccine of Salmonellaeen_US
dc.typeResearch Reportszh_TW
item.openairetypeResearch Reports-
item.fulltextno fulltext-
Appears in Collections:研究計畫報告
Show simple item record
TAIR Related Article

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.