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標題: Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor
作者: Hsu, S.Y.
Bartling, B.
Wang, C.
Shieu, F.S.
Liu, C.C.
關鍵字: diglyceride;disposable biosensor;enzyme;protein-kinase-c;dietary diacylglycerol;phospholipase-c;electrochemical biosensor;liquid-chromatography;dag diacylglycerol;mass-spectrometry;dimethyl ether;fatty-acids;cell
Project: Sensors
期刊/報告no:: Sensors, Volume 10, Issue 6, Page(s) 5758-5773.
A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 mu M in phosphate buffer and bovine serum media.
ISSN: 1424-8220
DOI: 10.3390/s100605758
Appears in Collections:工學院

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