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標題: Expression, crystallization and preliminary X-ray diffraction studies of N-carbamyl-D-amino-acid amidohydrolase from Agrobacterium radiobacter
作者: Hsu, W.H.
Chien, F.T.
Hsu, C.L.
Wang, T.C.
Yuan, H.S.
Wang, W.C.
關鍵字: purification
Project: Acta Crystallographica Section D-Biological Crystallography
期刊/報告no:: Acta Crystallographica Section D-Biological Crystallography, Volume 55, Page(s) 694-695.
The Agrobacterium radiobacter CCRC 14924 N-carbamyl-D-amino-acid amidohydrolase, the enzyme used for production of D-amino acids, was overexpressed in Escherichia coli JM109. The expressed protein was crystallized by vapour diffusion using lithium sulfate as precipitant. It crystallizes in space group P2(1) with unit-cell parameters cr = 69.8, b = 67.9 and c = 137.8 Angstrom and beta = 96.4 degrees. There are four molecules per asymmetric unit. Crystals diffract to 2.8 Angstrom resolution using a rotating-anode source at cryogenic (113 K) temperatures.
ISSN: 0907-4449
DOI: 10.1107/s090744499801525x
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