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標題: Ciprofloxacin and tetracycline susceptibility of lactobacilli isolated from indigenous children's feces
作者: Chang, Y.C.
Wang, Y.C.
Wang, I.K.
Lin, C.F.
Chuang, H.L.
Wu, Y.Y.
Chung, T.C.
Chen, T.H.
關鍵字: Indigenous child;ciprofloxacin and tetracycline resistance;lactobacilli;Lactobacilli-selective rogosa agar;cycloheximide;antimicrobial susceptibility test;repetitive extragenic palindromic;(REP)-PCR;minimum inhibitory concentration (MIC);intestinal microflora;antibiotic-resistance;identification;pig;bacteria;tract
Project: African Journal of Microbiology Research
期刊/報告no:: African Journal of Microbiology Research, Volume 6, Issue 2, Page(s) 245-250.
To investigate the frequency of ciprofloxacin and tetracycline resistance lactobacilli in children feces, a total of 160 feces samples were cultured on Lactobacilli-selective Rogosa agar supplemented with 0.1 mg/ml of cycloheximide and 0.5% of CaCO3, and identified Lactobacillus species were identified by analysis of the PCR sequenced-16S rRNA gene through BLAST against the deposited GenBank database. In these samples, 96 isolates were obtained and identified as belonging to 6 species, including Lactobacilli plantarum, Lactobacilli helveticusi, Lactobacilli salivarius, Lactobacilli casei, Lactobacilli fermentum and Lactobacilli pentosus. Strain-subtyping of these isolates by repetitive extragenic palindromic (REP)-PCR demonstrated a notable genotypic biodiversity of 65.6%. Antimicrobial susceptibility of ciprofloxacin and tetracycline had a wide different minimum inhibitory concentration (MIC) values in these isolates. The MIC50 and MIC90 of ciprofloxacin both was 64 mu g/ml for both, while the MIC50 and MIC90 of tetracycline were 128 and 512 mu g/ml. These results indicate that high-level resistant activity of ciprofloxacin and tetracycline among Lactobacillus species in indigenous children's intestines was prevalent in mountain district at the central area of Taiwan.
ISSN: 1996-0808
DOI: 10.5897/ajmr11.985
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