Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67857
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dc.contributor.authorCheng, C.C.en_US
dc.contributor.authorWu, L.C.en_US
dc.contributor.authorLai, J.M.en_US
dc.contributor.authorChen, C.T.en_US
dc.contributor.authorHsueh, C.M.en_US
dc.contributor.authorHsu, S.L.en_US
dc.date2012zh_TW
dc.date.accessioned2014-06-11T05:55:50Z-
dc.date.available2014-06-11T05:55:50Z-
dc.identifier.issn0192-415Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/67857-
dc.description.abstractHuman serum paraoxonase 1 (PON1), a calcium-dependent ester hydrolase, protects against the oxidative modification of low-density lipoprotein (LDL) and is a major anti-atherosclerotic component of high-density lipoprotein (HDL). Graptopetalum paraguayense, a folk herbal medicine commonly used in Taiwan, has antioxidative, anti-inflammatory, antihypertensive, and anti-atherogenic properties. The effects of G. paraguayense on the activity and/or expression of PON1 were examined using various extracts of the plant; extracts were made in water (GPWE), 50% ethanol (GP50E), and 95% ethanol (GP95E). Of these extracts, GP50E was found to be the most effective at increasing the function and expression of PON1 in a human hepatoma HepG2 cell line. Data from electrophoretic mobility shift assays and promoter-reporter luciferase analyses demonstrated that the DNA binding activity and transactivation ability of NF-kappa B were enhanced by GP50E. Treatment with NF-kappa B inhibitors, pyrrolidine dithiocarbamate, and BAY 11-7082 significantly attenuated GP50E-induced PON1 production and NF-kappa B transactivation activity. In addition, GP50E increased the levels of phosphorylated protein kinase B (PKB/AKT). Pharmacological inhibition of AKT by LY294002 effectively suppressed NF-kappa B activation and PON1 gene expression, suggesting that AKT was an upstream regulator of GP50E-mediated biological events. Overall, the results show that GP50E up-regulated PON1 gene expression via an AKT/NF kappa B-dependent signaling pathway in human hepatoma HepG2 cells. This observation led to the conclusion that the anti-atherogenic characteristics of G. paraguayense are modulated, at least in part, via the up-regulation of hepatocyte PON1 gene expression.en_US
dc.language.isoen_USzh_TW
dc.relationAmerican Journal of Chinese Medicineen_US
dc.relation.ispartofseriesAmerican Journal of Chinese Medicine, Volume 40, Issue 2, Page(s) 357-372.en_US
dc.relation.urihttp://dx.doi.org/10.1142/s0192415x12500280en_US
dc.subjectParaoxonase 1en_US
dc.subjectGraptopetalum paraguayenseen_US
dc.subjectNF-kappa Ben_US
dc.subjectAKTen_US
dc.subjectHepatomaen_US
dc.subjectCell Lineen_US
dc.subjectnecrosis-factor-alphaen_US
dc.subjectserum paraoxonaseen_US
dc.subjectdiabetes-mellitusen_US
dc.subjectprotein-kinaseen_US
dc.subjectwalther,e. extractsen_US
dc.subjecthepg2 cellsen_US
dc.subjectlipoproteinsen_US
dc.subjectactivationen_US
dc.subjectinductionen_US
dc.subjectatherosclerosisen_US
dc.titleEthanol Extract of Graptopetalum paraguayense Upregulates Paraoxonase 1 Gene Expression via an AKT/NF-kappa B-Dependent Pathwayen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1142/s0192415x12500280zh_TW
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
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