Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67874
標題: Characterization of tetracycline resistance lactobacilli isolated from swine intestines at western area of Taiwan
作者: Chang, Y.C.
Tsai, C.Y.
Lin, C.F.
Wang, Y.C.
Wang, I.K.
Chung, T.C.
關鍵字: Tetracycline resistance (Tet-R) lactobacilli;Swine;Minimum inhibitory;concentration (MIC);Tetracycline resistance determinants;lactic-acid bacteria;antibiotic-resistance;molecular characterization;antimicrobial resistance;paracasei strains;digestive-tract;fecal;samples;multiplex pcr;genes;identification
Project: Anaerobe
期刊/報告no:: Anaerobe, Volume 17, Issue 5, Page(s) 239-245.
摘要: 
To investigate the frequency of tetracycline resistance (Tet-R) lactobacilli in pig intestines, a total of 256 pig colons were analyzed and found to contain typical colonies of Tet-R lactic acid bacteria in every sample, ranging from 3.2 x 10(3) to 6.6 x 10(5) CFU/cm(2). From these samples, a total of 159 isolates of Tet-R lactobacilli were obtained and identified as belonging to 11 species, including Lactobacillus reuteri, Lactobacillus amylovorus, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus ruminis, Lactobacillus kefiri, Lactobacillus fermentum, Lactobacillus sakei, Lactobacillus coryniformis, Lactobacillus parabuchneri and Lactobacillus letivazi. Based on the EFSA (2008) breakpoints, all isolates, after MIC analysis, were qualified as Tet-R, from which the significant high Tet-R MIC(50) and MIC(90) values indicated an ecological distribution of Tet-R lactobacilli mostly with high resistance potency in pig colons. PCR-detection identified 5 tet genes in these isolates, the most predominant one being tet (W), followed by tet (M), (L), (K), and (Q). Their detection rates were 82.0%, 22.5%, 14.4%, 8.1% and 0.9%, respectively. Noteworthily, isolates of the same species carrying identical tet gene(s) usually had a wide different MIC values. Furthermore, strain-subtyping of these isolates by REP-PCR demonstrated a notable genotypic biodiversity % (average = 62%). (C) 2011 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/67874
ISSN: 1075-9964
DOI: 10.1016/j.anaerobe.2011.08.001
Appears in Collections:期刊論文

Show full item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.