Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67881
DC FieldValueLanguage
dc.contributor.authorJou, Y.J.en_US
dc.contributor.authorLin, C.D.en_US
dc.contributor.authorLai, C.H.en_US
dc.contributor.authorChen, C.H.en_US
dc.contributor.authorKao, J.Y.en_US
dc.contributor.authorChen, S.Y.en_US
dc.contributor.authorTsai, M.H.en_US
dc.contributor.authorHuang, S.H.en_US
dc.contributor.authorLin, C.W.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-11T05:55:52Z-
dc.date.available2014-06-11T05:55:52Z-
dc.identifier.issn0003-2670zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/67881-
dc.description.abstractOral cancer has a low five-year survival rate. Early detection of oral cancer could reduce the mortality and morbidity associated with this disease. Saliva, which can be sampled non-invasively and is less complex than blood, is a good potential source of oral cancer biomarkers. Proteomic analysis of saliva from oral cancer patients and control subjects was performed to identify salivary biomarkers of early stage oral cancer in humans. The protein profile of pooled salivary samples from patients with oral squamous cell carcinoma (OSCC) or OSCC-free control subjects was analyzed using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyses. Potential biomarkers were verified by Western blotting and ELISA assays. Transferrin levels were elevated in the saliva of OSCC patients as determined using 2DE followed by MALDI-TOF MS and confirmed by MALDI-TOF/TOF MS, Western blotting and ELISA. The increase in salivary transferrin levels in OSCC patients strongly correlated with the size and stage of the tumor. The area under the receiver-operating characteristics curves showed that salivary transferrin-based ELISA was highly specific, sensitive and accurate for the early detection of oral cancer. We have identified salivary transferrin as a biomarker for the detection of early stage oral cancer. This finding provides a promising basis for the development of a non-invasive diagnostic test for early stage oral cancer. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationAnalytica Chimica Actaen_US
dc.relation.ispartofseriesAnalytica Chimica Acta, Volume 681, Issue 1-2, Page(s) 41-48.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.aca.2010.09.030en_US
dc.subjectOral squamous cell carcinomaen_US
dc.subjectTwo-dimensional gel electrophoresisen_US
dc.subjectMatrix-assisted laser desorption/ionization time-of-flight massen_US
dc.subjectspectrometryen_US
dc.subjectSalivary transferrinen_US
dc.subjectReceiver-operating characteristicsen_US
dc.subjectcurveen_US
dc.subjectEarly detectionen_US
dc.subjectsquamous-cell carcinomaen_US
dc.subjectneck-canceren_US
dc.subjectreceptor expressionen_US
dc.subjectlung-canceren_US
dc.subjectheaden_US
dc.subjecttherapyen_US
dc.subjectdiagnosisen_US
dc.subjectproteinsen_US
dc.subjectmechanismsen_US
dc.subjectalcoholen_US
dc.titleProteomic identification of salivary transferrin as a biomarker for early detection of oral canceren_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.aca.2010.09.030zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
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