Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67898
DC FieldValueLanguage
dc.contributor.authorHuang, H.L.en_US
dc.contributor.authorCheng, Y.S.en_US
dc.contributor.authorHuang, C.W.en_US
dc.contributor.authorHuang, M.C.en_US
dc.contributor.authorHsu, W.H.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-11T05:55:54Z-
dc.date.available2014-06-11T05:55:54Z-
dc.identifier.issn0268-9146zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/67898-
dc.description.abstractTranscriptome analysis using a cDNA microarray was performed to identify differentially expressed genes that are correlated with hatchability, and a new PCR-RFLP marker of high hatchability among the identified genes was observed. We used the cDNA microarray technique for gene expression profiling of the magnum epithelium of laying Tsaiya ducks, and several regulated genes associated with hatchability were found. The results of real-time PCR and Western blotting analysis confirmed that the mRNA and protein levels of ovomucoid in the magnum epithelium of animals in the low-hatchability group were significantly higher than the levels in the high-hatchability group ( P < 0.05). Primers TovF1 and TovR1, designed according to the ovomucoid EST sequence, were used to amplify genomic DNA samples of different individual Tsaiya ducks, and sequence analysis of the amplified DNA products showed deletion among the ducks from the low-hatchability group. Primers TovF2 and TovR2 were used to perform PCR-RFLP analysis on the amplified DNA products to classify the ducks into +/+, +/- and -/- genotypes. The animals of +/+ and +/- genotypes were identified as having significantly higher hatchability than those of the -/- genotype ( P < 0.05). In contrast, no differences were observed between genotypes in terms of fertility, duration of fertility, egg weight or total number of eggs. Our results indicated that a novel PCR-RFLP marker of high hatchability, an ovomucoid gene polymorphism, can be used as a genetic marker for marker-assisted selection to improve hatchability in Tsaiya ducks.en_US
dc.language.isoen_USzh_TW
dc.relationAnimal Geneticsen_US
dc.relation.ispartofseriesAnimal Genetics, Volume 42, Issue 4, Page(s) 421-427.en_US
dc.relation.urihttp://dx.doi.org/10.1111/j.1365-2052.2010.02161.xen_US
dc.subjectcDNA microarrayen_US
dc.subjecthatchabilityen_US
dc.subjectmarker-assisted selectionen_US
dc.subjectovomucoiden_US
dc.subjectgeneen_US
dc.subjectTsaiya ducken_US
dc.subjectegg-whiteen_US
dc.subjectexpressed transcriptsen_US
dc.subjectbroiler breedersen_US
dc.subjectfertilityen_US
dc.subjectovalbuminen_US
dc.subjectlessonsen_US
dc.subjectalbuminen_US
dc.subjectproteinen_US
dc.subjectfamilyen_US
dc.subjectsemenen_US
dc.titleA novel genetic marker of the ovomucoid gene associated with hatchability in Tsaiya ducks (Anas platyrhynchos)en_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1111/j.1365-2052.2010.02161.xzh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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