Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/67905
DC FieldValueLanguage
dc.contributor.authorChen, C.H.en_US
dc.contributor.authorHuang, C.C.en_US
dc.contributor.authorChung, T.C.en_US
dc.contributor.authorHu, R.M.en_US
dc.contributor.authorHuang, Y.W.en_US
dc.contributor.authorYang, T.C.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-11T05:55:55Z-
dc.date.available2014-06-11T05:55:55Z-
dc.identifier.issn0066-4804zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/67905-
dc.description.abstractKJ09C, a multidrug-resistant mutant of Stenotrophomonas maltophilia KJ, was generated by in vitro selection with chloramphenicol. The multidrug-resistant phenotype of KJ09C was attributed to overexpression of a resistance nodulation division (RND)-type efflux system encoded by an operon consisting of five genes: smeU1, smeV, smeW, smeU2, and smeX. Proteins encoded by smeV, smeW, and smeX were similar to the membrane fusion protein, RND transporter, and outer membrane protein, respectively, of known RND-type systems. The proteins encoded by smeU1 and smeU2 were found to belong to the family of short-chain dehydrogenases/reductases. Mutant KJ09C exhibited increased resistance to chloramphenicol, quinolones, and tetracyclines and susceptibility to aminoglycosides; susceptibility to beta-lactams and erythromycin was not affected. The expression of the smeU1-V-W-U2-X operon was regulated by the divergently transcribed LysR-type regulator gene smeRv. Overexpression of the SmeVWX pump contributed to the acquired resistance to chloramphenicol, quinolones, and tetracyclines. Inactivation of smeV and smeW completely abolished the activity of the SmeVWX pump, whereas inactivation of smeX alone decreased the activity of the SmeVWX pump. The enhanced aminoglycoside susceptibility observed in KJ09C resulted from SmeX overexpression.en_US
dc.language.isoen_USzh_TW
dc.relationAntimicrobial Agents and Chemotherapyen_US
dc.relation.ispartofseriesAntimicrobial Agents and Chemotherapy, Volume 55, Issue 12, Page(s) 5826-5833.en_US
dc.relation.urihttp://dx.doi.org/10.1128/aac.00317-11en_US
dc.subjectgram-negative bacteriaen_US
dc.subjectpseudomonas-aeruginosaen_US
dc.subjectantibiotic-resistanceen_US
dc.subjectdrug-resistanceen_US
dc.subjectcomplete genomeen_US
dc.subjectsystemen_US
dc.subjectexpressionen_US
dc.subjectregulatoren_US
dc.subjectcloningen_US
dc.subjectsmedefen_US
dc.titleContribution of Resistance-Nodulation-Division Efflux Pump Operon smeU1-V-W-U2-X to Multidrug Resistance of Stenotrophomonas maltophiliaen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1128/aac.00317-11zh_TW
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
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