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標題: Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon TnMERI1
作者: Narita, M.
Yamagata, T.
Ishii, H.
Huang, C.C.
Endo, G.
關鍵字: class-ii transposon;mercury-resistance;escherichia-coli;methylmercury;outbreak;japan;iraq;mb1
Project: Applied Microbiology and Biotechnology
期刊/報告no:: Applied Microbiology and Biotechnology, Volume 59, Issue 1, Page(s) 86-90.
Using a newly identified organomercury lyase gene (merB3) expression system from TnMERII. the mercury resistance transposon first found in Gram-positive bacteria, a dual-purpose system to detect and remove organomercurial contamination was developed. A plasmid was constructed by fusing the promoterless lux-AB genes as bioluminescence reporter genes downstream of the merB3 gene and its operator/promoter region. Another plasmid, encoding mer operon genes from merR1 to merA, was also constructed to generate an expression regulatory protein, MerR1, and a mercury reductase enzyme, MerA. These two plasmids were transformed into Escherichia coli cells to produce a biological system that can detect and remove environmental organomercury contamination. Organomercurial compounds, such as neurotoxic methylmercury at nanomolar levels, were detected using the biomonitoring system within a few minutes and were removed during the next few hours.
ISSN: 0175-7598
DOI: 10.1007/s00253-002-0946-3
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