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http://hdl.handle.net/11455/68027
標題: | Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon TnMERI1 | 作者: | Narita, M. Yamagata, T. Ishii, H. Huang, C.C. Endo, G. |
關鍵字: | class-ii transposon;mercury-resistance;escherichia-coli;methylmercury;outbreak;japan;iraq;mb1 | Project: | Applied Microbiology and Biotechnology | 期刊/報告no:: | Applied Microbiology and Biotechnology, Volume 59, Issue 1, Page(s) 86-90. | 摘要: | Using a newly identified organomercury lyase gene (merB3) expression system from TnMERII. the mercury resistance transposon first found in Gram-positive bacteria, a dual-purpose system to detect and remove organomercurial contamination was developed. A plasmid was constructed by fusing the promoterless lux-AB genes as bioluminescence reporter genes downstream of the merB3 gene and its operator/promoter region. Another plasmid, encoding mer operon genes from merR1 to merA, was also constructed to generate an expression regulatory protein, MerR1, and a mercury reductase enzyme, MerA. These two plasmids were transformed into Escherichia coli cells to produce a biological system that can detect and remove environmental organomercury contamination. Organomercurial compounds, such as neurotoxic methylmercury at nanomolar levels, were detected using the biomonitoring system within a few minutes and were removed during the next few hours. |
URI: | http://hdl.handle.net/11455/68027 | ISSN: | 0175-7598 | DOI: | 10.1007/s00253-002-0946-3 |
Appears in Collections: | 期刊論文 |
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