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|標題:||Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon TnMERI1||作者:||Narita, M.
|關鍵字:||class-ii transposon;mercury-resistance;escherichia-coli;methylmercury;outbreak;japan;iraq;mb1||Project:||Applied Microbiology and Biotechnology||期刊/報告no：:||Applied Microbiology and Biotechnology, Volume 59, Issue 1, Page(s) 86-90.||摘要:||
Using a newly identified organomercury lyase gene (merB3) expression system from TnMERII. the mercury resistance transposon first found in Gram-positive bacteria, a dual-purpose system to detect and remove organomercurial contamination was developed. A plasmid was constructed by fusing the promoterless lux-AB genes as bioluminescence reporter genes downstream of the merB3 gene and its operator/promoter region. Another plasmid, encoding mer operon genes from merR1 to merA, was also constructed to generate an expression regulatory protein, MerR1, and a mercury reductase enzyme, MerA. These two plasmids were transformed into Escherichia coli cells to produce a biological system that can detect and remove environmental organomercury contamination. Organomercurial compounds, such as neurotoxic methylmercury at nanomolar levels, were detected using the biomonitoring system within a few minutes and were removed during the next few hours.
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