Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68150
DC FieldValueLanguage
dc.contributor.authorWang, S.Y.en_US
dc.contributor.authorTseng, C.P.en_US
dc.contributor.authorTsai, K.C.en_US
dc.contributor.authorLin, C.F.en_US
dc.contributor.authorWen, C.Y.en_US
dc.contributor.authorTsaye, H.S.en_US
dc.contributor.authorSakamoto, N.en_US
dc.contributor.authorTseng, C.H.en_US
dc.contributor.authorCheng, J.C.en_US
dc.date2009zh_TW
dc.date.accessioned2014-06-11T05:56:19Z-
dc.date.available2014-06-11T05:56:19Z-
dc.identifier.issn0006-291Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68150-
dc.description.abstractChronic hepatitis C virus (HCV) infection is a worldwide public issue. In this study, we performed bioactivity-guided screening of the Lonicera hypoglauca Miq. crude extracts to find for naturally chemical entities with anti-HCV activity. Pheophytin a was identified from the ethano-soluble fraction of L. hypoglauca that elicited dose-dependent inhibition of HCV vital proteins and RNA expression in both replicon cells and cell culture infectious system. Computational modeling revealed that pheophytin a can bind to the active site of HCV-NS3, suggesting that NS3 is a potent molecular target of pheophytin a. Biochemical analysis further revealed that pheophytin a inhibited NS3 serine protease activity with IC(50) = 0.89 mu M. Notably, pheophytin a and IFN alpha-2a elicited synergistic anti-HCV activity in replicon cells with no significant cytotoxicity. This study thereby demonstrates for the first time that pheophytin a is a potent HCV-NS3 protease inhibitor and offers insight for development of novel anti-HCV regimens. (C) 2009 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofseriesBiochemical and Biophysical Research Communications, Volume 385, Issue 2, Page(s) 230-235.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.bbrc.2009.05.043en_US
dc.subjectHepatitis C virusen_US
dc.subjectLonicera hypoglauca Miq.en_US
dc.subjectPheophytin aen_US
dc.subjectRepliconen_US
dc.subjectcellsen_US
dc.subjectserine-proteaseen_US
dc.subjectrna replicationen_US
dc.subjectcell-cultureen_US
dc.subjectinhibitionen_US
dc.subjectinfectionen_US
dc.subjectribavirinen_US
dc.subjectvariantsen_US
dc.subjectjaponicaen_US
dc.subjectchineseen_US
dc.subjectsystemen_US
dc.titleBioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miqen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.bbrc.2009.05.043zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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