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標題: Molecular phylogeny of the Pycnonotus sinensis and Pycnonotus taivanus in Taiwan based on sequence variations of nuclear CHD and mitochondrial cytochrome b genes
作者: Chang, H.W.
Chou, Y.C.
Su, Y.F.
Cheng, C.A.
Yao, C.T.
Tsai, C.L.
Lee, H.C.
Wen, C.H.
Cheng, C.C.
關鍵字: Chinese Bulbul;CHD-Z;CHD-W;cytochrome b;SNP;Taiwan Bulbul;gender identification;evolutionary genetics;bayesian-inference;dna-sequences;cyt-b;aves;woodpeckers;cyprinidae;eagles;region
Project: Biochemical Systematics and Ecology
期刊/報告no:: Biochemical Systematics and Ecology, Volume 38, Issue 2, Page(s) 195-201.
Sequences of the partial 293-bp nuclear Z-chromosome-linked chromo-helicase binding protein (CHD-Z) and 729-bp mitochondrial cytochrome b (cyt b) genes were obtained from two species of the same family Pycnonotidae of Pycnonotus sinensis (P. sinensis, Chinese Bulbul) and Pycnonotus taivanus (P. taivanus, Taiwan Bulbul) distributed in Taiwan. A panel of 10 individuals (n = 5 for each) with unknown relationship was used to characterize single nucleotide polymorphisms (SNPs) of these genes. We identified 2 and 10 SNPs in CHD-Z and cyt b loci, respectively. Frequency of SNPs was 10 per every 1465- and 729-bp on average. Pairwise nucleotide divergences of CHD-Z and cyt b genes among 10 specimens ranged from 0 to 0.0034 and 0 to 0.0055, respectively. Phylogenetic analysis suggested that the P taivanus group assignment based on the CHD-Z and cyt b sequences is obviously very similar to P. sinensis. (C) 2009 Elsevier Ltd. All rights reserved.
ISSN: 0305-1978
DOI: 10.1016/j.bse.2009.12.016
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