Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68213
DC FieldValueLanguage
dc.contributor.authorChen, Y.C.en_US
dc.contributor.authorLee, D.C.en_US
dc.contributor.authorTsai, T.Y.en_US
dc.contributor.authorHsiao, C.Y.en_US
dc.contributor.authorLiu, J.W.en_US
dc.contributor.authorKao, C.Y.en_US
dc.contributor.authorLin, H.K.en_US
dc.contributor.authorChen, H.C.en_US
dc.contributor.authorPalathinkal, T.J.en_US
dc.contributor.authorPong, W.F.en_US
dc.contributor.authorTai, N.H.en_US
dc.contributor.authorLin, I.N.en_US
dc.contributor.authorChiu, I.M.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-11T05:56:26Z-
dc.date.available2014-06-11T05:56:26Z-
dc.identifier.issn0142-9612zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68213-
dc.description.abstractThe interaction of ultra-nanocrystalline diamond (UNCD) with neural stem cells (NSCs) has been studied in order to evaluate its potential as a biomaterial. Hydrogen-terminated UNCD (H-UNCD) films were compared with standard grade polystyrene in terms of their impact on the differentiation of NSCs. When NSCs were cultured on these substrates in medium supplemented with low concentration of serum and without any differentiating factors, H-UNCD films spontaneously induced neuronal differentiation on NSCs. By direct suppression of mitogen-activated protein kinase/extracellular signaling-regulated kinase1/2 (MAPK/Erk1/2) signaling pathway in NSCs using U0126, known to inhibit the activation of Erk1/2, we demonstrated that the enhancement of Erk1/2 pathway is one of the effects of H-UNCD-induced NSCs differentiation. Moreover, functional-blocking antibody directed against integrin beta 1 subunit inhibited neuronal differentiation on H-UNCD films. This result demonstrated the involvement of integrin beta 1 in H-UNCD-mediated neuronal differentiation. Mechanistic studies revealed the cell adhesion to H-UNCD films associated with focal adhesion kinase (Fak) and initiated MAPK/Erk1/2 signaling. Our study demonstrated that H-UNCD films-mediated NSCs differentiation involves fibronectin-integrin beta 1 and Fak-MAPK/Erk signaling pathways in the absence of differentiation factors. These observations raise the potential for the use of UNCD as a biomaterial for central nervous system transplantation and tissue engineering. (c) 2010 Elsevier Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationBiomaterialsen_US
dc.relation.ispartofseriesBiomaterials, Volume 31, Issue 21, Page(s) 5575-5587.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.biomaterials.2010.03.061en_US
dc.subjectUltra-nanocrystalline diamonden_US
dc.subjectNeural stem cellsen_US
dc.subjectDifferentiationen_US
dc.subjectcentral-nervous-systemen_US
dc.subjectstem/progenitor cellsen_US
dc.subjectneurotrophic factorsen_US
dc.subjectsignaling pathwayen_US
dc.subjectmesenchymal stemen_US
dc.subjectprecursor cellsen_US
dc.subjectmap kinaseen_US
dc.subjectgrowthen_US
dc.subjectadhesionen_US
dc.subjectintegrinsen_US
dc.titleInduction and regulation of differentiation in neural stem cells on ultra-nanocrystalline diamond filmsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.biomaterials.2010.03.061zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
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