Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68219
DC FieldValueLanguage
dc.contributor.authorChang, J.C.en_US
dc.contributor.authorHsu, S.H.en_US
dc.contributor.authorChen, D.C.en_US
dc.date2009zh_TW
dc.date.accessioned2014-06-11T05:56:27Z-
dc.date.available2014-06-11T05:56:27Z-
dc.identifier.issn0142-9612zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68219-
dc.description.abstractThe dynamic regulation of integrin-binding peptides is crucial for chondrogenic differentiation. Here, we revealed the feasibility for flexible modification of RGD by embedding a large molecular weight and slightly charged (isoelectric point, 6-6.25) RGD-chimeric protein (CBD-RGD) with cellulose-binding domain (CBD) in three dimensional (3D) alginate beads to evaluate the chondrogenesis of adipose-derived adult stem cells (ADAS). The binding of CBD-RGD with cells and its diffusion from alginate beads were studied on fluorescein isothiocyanate (FITC)-conjugated CBD-RGD. The increases in gene expression (Sox9, Aggrecan, fibronectin and collagen II), accumulation of chondrogenic matrices and decrease of collagen X gene expression during TGF-beta 3 induction were only observed for those beads containing 10 mg/g CBD-RGD initially, with 20.18 +/- 0.73% of that released in a week. The contrary was observed for beads with CBD-RGD 20 mg/g initially and having higher persistence (only 8.6 +/- 2.17% released in a week). The 10 mg/g CBD-RGD-mediated enhancement was demonstrated via the activation of integrin alpha 5 and beta 1-dependent pathway, and especially related to the upregulation of Sox9 gene and the temporary block of fibronectin expression as well as sustained inhibition of RhoA activity in the early differentiation stage. Thus, we speculated that the dynamic mobility of CBD-RGD may account for the enhanced chondrogenesis. It was concluded that the CBD-RGD-alginate culture system promoted the chondrogenesis of mesenchymal stem cells coordinated with TGF-beta 3 induction in an RGD close-dependent manner. (C) 2009 Elsevier Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationBiomaterialsen_US
dc.relation.ispartofseriesBiomaterials, Volume 30, Issue 31, Page(s) 6265-6275.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.biomaterials.2009.07.064en_US
dc.subjectRGD-chimeric proteinen_US
dc.subjectAlginateen_US
dc.subjectAdipose-derived adult stem cellsen_US
dc.subjectChondrogenesisen_US
dc.subjectFibronectinen_US
dc.subjectRhoA activityen_US
dc.subjectvascular graftsen_US
dc.subjectfibronectinen_US
dc.subjecttissueen_US
dc.subjectdifferentiationen_US
dc.subjectexpressionen_US
dc.subjectbindingen_US
dc.subjectchondrocytesen_US
dc.subjectspecificityen_US
dc.subjectreceptorsen_US
dc.subjectscaffoldsen_US
dc.titleThe promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate cultureen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.biomaterials.2009.07.064zh_TW
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.grantfulltextnone-
Appears in Collections:期刊論文
Show simple item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.