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標題: Evaluation of Resveratrol Oxidation in Vitro and the Crucial Role of Bicarbonate Ions
作者: Yang, N.C.
Lee, C.H.
Song, T.Y.
關鍵字: bicarbonate ion;degradation;epigallocatechin-3-gallate (EGCG);oxidation;resveratrol;tea polyphenol (-)-epigallocatechin-3-gallate;hydrogen-peroxide;catechins;generation;quercetin;stability;molecule;gallate
Project: Bioscience Biotechnology and Biochemistry
期刊/報告no:: Bioscience Biotechnology and Biochemistry, Volume 74, Issue 1, Page(s) 63-68.
Polyphenols can oxidize in culture medium and produce artifacts in cell culture studies. However, the extent and mechanism of the oxidation of resveratrol, a polyphenol abundant in red wine, is unclear. We investigated the oxidation of resveratrol in vitro and the effects of various components of the culture medium on the degradation of resveratrol and the production of H(2)O(2). We found that 96% of resveratrol at a concentration of 200 mu M was degraded in Base Modified Eagle Medium after 24h of incubation at 37 degrees C, producing about 90 mu M of H(2)O(2). Including sodium bicarbonate in the medium markedly stimulated resveratrol degradation and H(2)O(2) production. In sum, we found that bicarbonate ions played a crucial role in the oxidative degradation of resveratrol in vitro, and that the degradation of resveratrol can be avoided by withdrawing sodium bicarbonate from the medium. A mechanism for the oxidation of resveratrol is proposed.
ISSN: 0916-8451
DOI: 10.1271/bbb.90549
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