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標題: Overexpression of a single membrane component from the Bacillus mer operon enhanced mercury resistance in an Escherichia coli host
作者: Hsieh, J.L.
Chen, C.Y.
Chang, J.S.
Endo, G.
Huang, C.C.
關鍵字: MerP;mercury;bioremediation;ion-binding protein;class-ii transposon;p-type atpase;metal-binding;bacterial resistances;cysteine residues;heavy-metals;expression;peptides;cells
Project: Bioscience Biotechnology and Biochemistry
期刊/報告no:: Bioscience Biotechnology and Biochemistry, Volume 71, Issue 6, Page(s) 1494-1499.
Overexpression of a mercuric ion binding protein, MerP, from the mercury resistance operon genes of Gram-positive bacterial strain Bacillus megaterium MB1 and from Gram-negative bacterial strain Pseudomonas aeruginosa K-62 was found to enhance the mercury resistance level of Escherichia coli host cells, even though they share only 27.3% identity. Immunoblot analysis showed that MerP (BMerP) from Bacillus could be expressed on the membrane fraction of E. coli cells. Treated with 10 mu M Hg2+, a recombinant strain harboring the BMerP gene significantly improved, showing a 27% increase in mercuric ion adsorption capacity, 16% better than that of a Pseudomonas merP gene (PMerP)-harboring strain. While multiple heavy metals co-existed, the mercuric ion adsorption capacity of the BMerP-harboring E. coli was not affected while that of the PMerP-harboring strain decreased. These results suggest that BMerP can act as a bio-adsorbent compartmentalizing the toxic mercuric ion on the cell membrane and enhancing resistance.
ISSN: 0916-8451
DOI: 10.1271/bbb.70003
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