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標題: A thermally baffled device for highly stabilized convective PCR
作者: Chang, H.F.G.
Tsai, Y.L.
Tsai, C.F.
Lin, C.K.
Lee, P.Y.
Teng, P.H.
Su, C.
Jeng, C.C.
關鍵字: Methods;Rayleigh-Benard convective PCR;Thermally baffled PCR;instrument;Yellow head virus;diagnosis;amplification;pathogens;cell
Project: Biotechnology Journal
期刊/報告no:: Biotechnology Journal, Volume 7, Issue 5, Page(s) 662-666.
Rayleigh-Benard convective PCR is a simple and effective design for amplification of DNA. Convective PCR is, however, extremely sensitive to environmental temperature fluctuations, especially when using small- diameter test tubes. Therefore, this method is inherently unstable with limited applications. Here, we present a convective PCR device that has been modified by adding thermal baffles. With this thermally baffled device the influence from fluctuations in environmental temperature were significantly reduced, even in a wind tunnel (1 m/s). The thermally baffled PCR instrument described here has the potential to be used as a low-cost, point-of-care device for PCR-based molecular diagnostics in the field.
ISSN: 1860-6768
DOI: 10.1002/biot.201100453
Appears in Collections:期刊論文

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