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標題: Ribosome inactivating protein B-chain induces osteoclast differentiation from monocyte/macrophage lineage precursor cells
作者: Wang, Y.M.
Lu, T.L.
Hsu, P.N.
Tang, C.H.
Chen, J.H.
Liu, K.C.
Kao, J.T.
Tzen, J.T.C.
Wu, Y.Y.
Project: Bone
期刊/報告no:: Bone, Volume 48, Issue 6, Page(s) 1336-1345.
Human osteoclast formation from mononuclear phagocyte precursors involves interactions between lectins and their receptors. A type-2 ribosome inactivating protein consists of an A chain and a B chain. The glycosylated B chain binds specifically to galactose moieties of sugar molecules. In this study we showed that the recombinant ribosome inactivating protein B-chain (rRBC) could induce osteoclast formation from human monocytes and murine RAW264.7 macrophages. Tartrate-resistant acid phosphatase (TRAP) staining and bone resorption assays demonstrated that differentiation of osteoclast-like cells was induced in the presence of rRBC in a dose-dependent manner. The rRBC-induced osteoclast differentiation was independent of caspase activation and apoptosis induction activity; however, rRBC-induced osteoclastogenesis was dependent on activation of NF-kappa B, ERK1/2, and p38 MAP kinase. Thus, our data demonstrated that rRBC induced osteoclast differentiation through a non-apoptotic signaling pathway. In addition to triggering apoptosis, the rRBC also induced osteoclast differentiation. According to this study, a novel role is proposed for rRBC in regulating osteoclast differentiation and in osteoimmunology. (C) 2011 Elsevier Inc. All rights reserved.
ISSN: 8756-3282
DOI: 10.1016/j.bone.2011.02.018
Appears in Collections:期刊論文

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