Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68314
DC FieldValueLanguage
dc.contributor.authorChan, P.C.en_US
dc.contributor.author陳鴻震zh_TW
dc.contributor.authorLai, J.F.en_US
dc.contributor.authorCheng, C.H.en_US
dc.contributor.authorTang, M.J.en_US
dc.contributor.authorChiu, C.C.en_US
dc.contributor.authorChen, H.C.en_US
dc.date1999zh_TW
dc.date.accessioned2014-06-11T05:56:37Z-
dc.date.available2014-06-11T05:56:37Z-
dc.identifier.issn0021-9258zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68314-
dc.description.abstractFocal adhesion kinase (FAK) has been implicated tea play a role in suppression of apoptosis. In this study, we have demonstrated that UV irradiation induced cleavage of FAK and two of its interacting proteins Src and p130(Cas) in Madin-Darby canine kidney cells, concomitant with an increase in cell death. The cleavage of these proteins upon UV irradiation was completely inhibited by ZVAD-FMK, a broad range inhibitor of caspases, and apparently delayed by Bcl2 overexpression. To examine if FAK plays a role in suppressing UV-induced apoptosis, stable Madin-Darby canine kidney cell lines overexpressing FAK were established. Our results showed that a marked (30-40%) increase in cell survival upon UV irradiation was achieved by this strategy. In our efforts to determine the mechanism by which FAK transduces survival signals to the downstream, we found that a PAK mutant deficient in binding to phosphatidylinositol 3-kinase failed to promote cell survival. Moreover, the expression of the Src homology 3 domain of p130(Cas), which competed with endogenous p130(Cas) for FAK binding, abrogated the FAK-promoted cell survival. Together, these results suggest that the integrity of FAK and its binding to phosphatidylinositol 3-kinase and p130(Cas) are required for FAK to exert its antiapoptotic function.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Biological Chemistryen_US
dc.relation.ispartofseriesJournal of Biological Chemistry, Volume 274, Issue 38, Page(s) 26901-26906.en_US
dc.subjectphosphotyrosine-containing proteinsen_US
dc.subjecttyrosine kinasesen_US
dc.subjectgrowth-factoren_US
dc.subjectphosphatidylinositol 3-kinaseen_US
dc.subjectsignal-transductionen_US
dc.subjectstable associationen_US
dc.subjectv-srcen_US
dc.subjectbindingen_US
dc.subjectactivationen_US
dc.subjectpp125(fak)en_US
dc.titleSuppression of ultraviolet irradiation-induced apoptosis by overexpression of focal adhesion kinase in Madin-Darby canine kidney cellsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1074/jbc.274.38.26901zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
Appears in Collections:生命科學院
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