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|標題:||Cloning and characterization of a cDNA encoding the endo-1,4-beta-D-glucanase gene expressed in rapidly growing tuber and leaf of taro (Colocasia esculenta var. esculenta)||作者:||Lin, D.G.
|關鍵字:||Cell wall assembly and taro (Colocasia esculenta var. esculenta);Endo-1,4-beta-glucanases;KORRIGAN;Rapid elongation;arabidopsis-thaliana;messenger-rna;cellulose synthesis;endo-1,4-beta-glucanase cel1;glycosyl hydrolases;escherichia-coli;plants;abscission;korrigan;elongation||Project:||Botanical Studies||期刊/報告no：:||Botanical Studies, Volume 50, Issue 4, Page(s) 379-387.||摘要:||
A cDNA for endo-beta-1,4-glucanase (EGase) was isolated by RT-PCR, and rapid amplification of cDNA ends reaction from taro leaves (Colocasia esculenta var. esculenta). This single copy gene has 2,185 bps and an open reading frame (ORF) of 1,854 bps. A polypeptide of 618 residues was deduced from the ORF, with a calculated molecular mass of 68,434 Da and theoretical pI of 8.8. The remarked activities of the EGase on CMC plate revealed this enzyme to be involved in cellulose metabolism in taro. Taro EGase was identified as a kind of membrane-anchored EGase. Phylogenetic analysis showed it is a member of the gamma subfamily and an ortholog of the Arabidopsis KORRIGAN gene (KOR), proposed to be involved in cytokinesis, pectin metabolism in the primary cell wall, and cell elongation. Transcripts of taro EGase are highly accumulated in 300 g tubers, in the upper part of 1,000 g tubers, and in rolled leaves undergoing rapid growth, indicating that taro EGase is involved in the regulation of plant growth. The expression pattern of soluble starch synthase III was similar to that of EGase during rapid growth of taro plant tubers. These results suggested that taro EGase, an ortholog of KOR in Arabidopsis, may play an important role in the rapid growth in taro.
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