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標題: Tyrosine phosphorylation of focal adhesion kinase stimulated by hepatocyte growth factor leads to mitogen-activated protein kinase activation
作者: Chen, H.C.
Chan, P.C.
Tang, M.J.
Cheng, C.H.
Chang, T.J.
關鍵字: cell-matrix interactions;src family kinases;swiss 3t3 cells;factor-receptor;signal-transduction;map kinase;c-met;phosphatidylinositol 3-kinase;complex-formation;adapter protein
Project: Journal of Biological Chemistry
期刊/報告no:: Journal of Biological Chemistry, Volume 273, Issue 40, Page(s) 25777-25782.
Focal adhesion kinase (FAK) is a cytoplasmic tyrosine kinase involved in integrin-mediated signal transduction pathway. In this report, we describe that the treatment of hepatocyte growth factor (HGF) stimulates a significant increase in the tyrosine phosphorylation of FAK in human embryonic kidney 293 cells. This stimulation is independent of cell adhesion or the integrity of the actin cytoskeleton, suggesting potentially different mechanisms by which the HGF receptors and integrins regulate the tyrosine phosphorylation of FAK. Our results also suggest that the activation of Src upon HGF stimulation is likely to be one, if not the only, of the mechanisms responsible for the HGF-induced tyrosine phosphorylation of FAK. Furthermore, we showed that a mutation in the Grb2 binding site Tyr-925 of FAK partially abolishes its increase in HGF-induced phosphorylation. Finally, we demonstrated that HGF stimulates the association of FAK with Grb2 in vitro and in intact cells and provided evidence that FAK might contribute to the activation of mitogen-activated protein kinase through Ras in HGF signaling by functioning as an adapter molecule.
ISSN: 0021-9258
DOI: 10.1074/jbc.273.40.25777
Appears in Collections:生命科學院

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