Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68431
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dc.contributor.authorWang, H.H.en_US
dc.contributor.authorYeh, H.I.en_US
dc.contributor.authorWang, C.Y.en_US
dc.contributor.authorSu, C.H.en_US
dc.contributor.authorWu, Y.J.en_US
dc.contributor.authorTseng, Y.Y.en_US
dc.contributor.authorLin, Y.C.en_US
dc.contributor.authorTsai, C.H.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-11T05:56:48Z-
dc.date.available2014-06-11T05:56:48Z-
dc.identifier.issn0742-2091zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68431-
dc.description.abstractWe investigated the effect of C-reactive protein (CRP) and sodium azide (NaN(3)) on endothelial Cx43 gap junctions. Human aortic endothelial cells (HAEC) were treated with (a) detoxified CRP, (b) detoxified dialyzed CRP, (c) detoxified dialyzed CRP plus NaN(3), (d) NaN(3), or (e) dialyzed NaN(3). The concentration of CRP in all preparations was fixed to 25 mu g/ml and that of NaN(3) in the preparations of (c) to (e) was equivalent to that contained in the 25 mu g/ml CRP purchased commercially. The results showed that both the expression of Cx43 protein and gap junctional communication function post-48-h incubation were reduced and inhibited by the detoxified CRP, NaN(3), or detoxified dialyzed CRP plus NaN(3), but not by the detoxified dialyzed CRP or dialyzed NaN(3). Reverse transcription-polymerase chain reaction analysis of cells treated for 72 h also showed a pattern of transcriptional regulation essentially the same as that for the proteins. We concluded that CRP does not have a significant effect on Cx43 gap junctions of HAEC, but NaN(3) inhibited the viability of cells and downregulate their junctions.en_US
dc.language.isoen_USzh_TW
dc.relationCell Biology and Toxicologyen_US
dc.relation.ispartofseriesCell Biology and Toxicology, Volume 26, Issue 2, Page(s) 153-163.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s10565-009-9125-yen_US
dc.subjectConnexin43en_US
dc.subjectC-reactive proteinen_US
dc.subjectEndothelial cellsen_US
dc.subjectGap junctionen_US
dc.subjectSodiumen_US
dc.subjectazideen_US
dc.subjectactivator inhibitor-1 expressionen_US
dc.subjectin-vitroen_US
dc.subjectintercellular communicationen_US
dc.subjectgene-expressionen_US
dc.subjectcellsen_US
dc.subjectdysfunctionen_US
dc.subjectatherothrombosisen_US
dc.subjectreleaseen_US
dc.subjectpathwayen_US
dc.subjectalphaen_US
dc.titleC-reactive protein, sodium azide, and endothelial connexin43 gap junctionsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s10565-009-9125-yzh_TW
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
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