Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68519
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dc.contributor.authorJou, Y.J.en_US
dc.contributor.authorLin, C.D.en_US
dc.contributor.authorLai, C.H.en_US
dc.contributor.authorTang, C.H.en_US
dc.contributor.authorHuang, S.H.en_US
dc.contributor.authorTsai, M.H.en_US
dc.contributor.authorChen, S.Y.en_US
dc.contributor.authorKao, J.Y.en_US
dc.contributor.authorLin, C.W.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-11T05:56:55Z-
dc.date.available2014-06-11T05:56:55Z-
dc.identifier.issn0009-8981zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68519-
dc.description.abstractBackground: Oral squamous cell carcinoma (OSCC) is one of the most frequent malignancies worldwide. Early diagnosis can mean adequate treatment and increase survival. Methods: This study uses ClinProt technique to identify salivary biomarkers for early diagnosis of OSCC. A total of 77 salivary samples from both OSCC patients (n = 47) and healthy donors (n = 30) were analyzed with MALDI-TOF MS technology. Results: Salivary peptides from OSCC patients were separated, using C8-functionalized magnetic beads. Three signals (2918.57 Da, 5592.64 Da, and 4372.66 Da) distinguished OSCC patients from controls. Among them, unique peptide 2918.57 Da, identified as a 24-mer peptide of zinc finger protein 510 (ZNF510). was found in 0% of saliva from healthy individuals, versus 25.0% and 60% from OSCC patients with T1 + T2 and T3 + T4 stages, respectively (P < 0.001). ELISA analysis with rabbit anti-ZNF510 peptide sera shows a starkly higher 24-mer ZNF510 peptide level in saliva from OSCC patients than that in controls (P<0.001). Also, in immunohistochemical analysis of oral tissues, a significantly higher level of ZNF510 was observed in OSCC tissues than in the OSCC free control tissues. Analysis of areas under receiver-operating characteristic (ROC) curves in OSCC early (T1 + T2) and late stages (T3 + T4) shows greater than 0.95. Conclusions: Identifying 24-met ZNF510 peptide as OSCC-related salivary biomarkers via proteomic approach proved useful in adjunct diagnosis for early detection rather than specific diagnosis marker for progression of OSCC patients. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationClinica Chimica Actaen_US
dc.relation.ispartofseriesClinica Chimica Acta, Volume 412, Issue 15-16, Page(s) 1357-1365.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.cca.2011.04.004en_US
dc.subjectOral squamous cell carcinomaen_US
dc.subjectBiomarkersen_US
dc.subjectMatrix-assisted laseren_US
dc.subjectdesorption/ionization time-of-flighten_US
dc.subjectZinc finger protein 510en_US
dc.subjectClinProten_US
dc.subjectneck-canceren_US
dc.subjectdiagnosisen_US
dc.subjectznf652en_US
dc.subjectheaden_US
dc.titleSalivary zinc finger protein 510 peptide as a novel biomarker for detection of oral squamous cell carcinoma in early stagesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.cca.2011.04.004zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
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