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標題: Screening of compactin-resistant microorganisms capable of converting compactin to pravastatin
作者: Chen, C.H.
Hu, H.Y.
Cho, Y.C.
Hsu, W.H.
關鍵字: tissue-selective inhibitor;coenzyme-a reductase;ml-236b compactin;hydroxylation;cs-514
Project: Current Microbiology
期刊/報告no:: Current Microbiology, Volume 53, Issue 2, Page(s) 108-112.
A simple method of using compactin for effective screening of microbial strains with high hydroxylation activity at the 6 beta position of compactin was developed. Agar plates containing different carbon sources and 500 mu g compactin mL(-1) were used to screen the microorganisms that can convert compactin to pravastatin. About 100 compactin-resistant strains were isolated from the Basal agar containing 7% (w/v) mannitol as a carbon source, in which two bacteria, Pseudomocardia autotrophica BCRC 12444 and Streptomyces griseolus BCRC 13677, capable of converting compactin to pravastatin with the yield of 20 and 32% (w/w), respectively, were found. High-performance liquid chromatography using C-18 column and two sequential mobile phases, 30% and 50% (v/v) acetonitrile, was also established to simultaneously determine the concentration of compactin and pravastatin in the culture broth. As such, about 2% of target microorganisms could be obtained from the screening program.
ISSN: 0343-8651
DOI: 10.1007/s00284-005-0276-7
Appears in Collections:期刊論文

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