Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68650
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dc.contributor.authorHsu, S.H.en_US
dc.contributor.authorChang, J.C.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-11T05:57:07Z-
dc.date.available2014-06-11T05:57:07Z-
dc.identifier.issn0920-9069zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68650-
dc.description.abstractDental pulp cells (DPCs) can differentiate into osteoblasts and are deemed a promising cell source for bone regeneration. Static magnetic field (SMF) stimulates osteoblast differentiation but the effect in DPCs remains unknown. The aim of this study was to investigate the effect of SMF exposure on the osteogenic differentiation and mineralization of rat DPCs in vitro. Cells were continuously exposed to SMF at 290 mT in the presence/absence of osteogenic induction [dexamethasone (Dex)/beta-glycerophosphate (beta-GP)]. Results showed that SMF alone did not impair the cell cycle and proliferation. On the other hand, obvious condensation in the metachromatic staining of the extracellular matrix with toluidine blue was observed for SMF-exposed cells as well as the Dex/beta-GP treated cells. SMF in combination with Dex/beta-GP significantly increased the mRNA expression of osteogenic genes, as well as the ALP activity and extracellular calcium concentration at the early stage, followed by obvious calcium deposits later. Besides, SMF exposure increased the activity of extracellular signal-regulated kinase 1/2 (ERK1/2) at 3 h and accelerated the mRNA expression of osteogenic transcription factor, Cbfa1, advancing its activation time from 168 to 72 h under osteogenic induction. In summary, SMF exposure in combination of Dex/beta-GP induction could significantly accelerate the osteogenic differentiation and mineralization of DPCs.en_US
dc.language.isoen_USzh_TW
dc.relationCytotechnologyen_US
dc.relation.ispartofseriesCytotechnology, Volume 62, Issue 2, Page(s) 143-155.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s10616-010-9271-3en_US
dc.subjectStatic magnetic fielden_US
dc.subjectDental pulp cellsen_US
dc.subjectDexamethasoneen_US
dc.subjectGeneen_US
dc.subjectexpressionen_US
dc.subjectMineralizationen_US
dc.subjectpulsed electromagnetic-fieldsen_US
dc.subjectalkaline-phosphatase activityen_US
dc.subjectosteoblast-like cellsen_US
dc.subjectstem-cellsen_US
dc.subjectin-vitroen_US
dc.subjectbone-formationen_US
dc.subjecttranscription factoren_US
dc.subjectgene-expressionen_US
dc.subjectraten_US
dc.subjectexposureen_US
dc.titleThe static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cellsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s10616-010-9271-3zh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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