Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68672
DC FieldValueLanguage
dc.contributor.authorTsai, C.C.en_US
dc.contributor.authorChen, S.Y.en_US
dc.contributor.authorTsen, H.Y.en_US
dc.date2003zh_TW
dc.date.accessioned2014-06-11T05:57:09Z-
dc.date.available2014-06-11T05:57:09Z-
dc.identifier.issn0732-8893zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68672-
dc.description.abstractEnteroaggregative Escherichia coli (EAggEC) are emerging enteropathogens associated with human diarrhea diseases and food poisoning cases. They show distinctive aggregative pattern of adherence to cultured human epithelial cells. However, EAggEC strains are diverse and not all of them have the aggregative adherence fimbria I (AAF/1), AAF/II and heat-stable enterotoxin 1 (EAST1) genes. We attemptd to determine the incidence of EAggEC in E. coli isolates from diarrhea patients in Taiwan and to characterize these EAggEC strains. We used three activity assays including HeLa, cell adhesion, human blood hemagglutination and bacterial clumping tests and polymerase chain reaction (PCR) primers designed from an aggregative adherence pattern associated plasmid (pCVD432) to screen the EAggEC strains in 403 E. coli isolates including 63 laboratory isolates and 340 clinical isolates obtained from diarrheal disease cases. All these 403 E. coli strains were also assayed with novel PCR primers designed from AAF/I (aggA), AAF/II (aafA) and EAST I (astA) genes. Results showed that except for the three EAggEC reference strains, only three clinical isolates were identified as EAggEC strains. Including the reference strains, all the E. coli strains with EAggEC activity generated positive PCR results to the aggA gene based primers, but not to the aafA and astA gene targeted primers. (C) 2003 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationDiagnostic Microbiology and Infectious Diseaseen_US
dc.relation.ispartofseriesDiagnostic Microbiology and Infectious Disease, Volume 46, Issue 3, Page(s) 159-165.en_US
dc.relation.urihttp://dx.doi.org/10.1016/s0732-8893(03)00043-9en_US
dc.subjectenteroaggregative Escherichia colien_US
dc.subjectadhesionen_US
dc.subjecthemagglutinationen_US
dc.subjectbacterial clumping testen_US
dc.subjectPCRen_US
dc.subjectbacterial clump formationen_US
dc.subjectpolymerase-chain-reactionen_US
dc.subjectheat-stableen_US
dc.subjectenterotoxinen_US
dc.subjectadherence fimbria-iien_US
dc.subjectaggregative adherenceen_US
dc.subjecthep-2 cellsen_US
dc.subjectenteroadherenten_US
dc.subjecthemagglutinationen_US
dc.subjectidentificationen_US
dc.subjectprevalenceen_US
dc.titleScreening the enteroaggregative Escherichia coli activity and detection of the aggA, aafA, and astA genes with novel PCR primers for the Escherichia coli isolates from diarrhea cases in Taiwanen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/s0732-8893(03)00043-9zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
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