Please use this identifier to cite or link to this item:
|標題:||Ceramide accumulation and up-regulation of proinflammatory interleukin-1 beta exemplify lipotoxicity to mediate declines of reproductive efficacy of broiler hens||作者:||Pan, Y.E.
|關鍵字:||Broiler chickens;Ceramide;Interleukin-1 beta;Follicle atresia;Lipotoxicity;meat-type chickens;high-fat diet;granulosa-cells;induced apoptosis;gallus-domesticus;ovarian-follicles;food restriction;body-weight;in-vitro;leptin||Project:||Domestic Animal Endocrinology||期刊/報告no：:||Domestic Animal Endocrinology, Volume 42, Issue 3, Page(s) 183-194.||摘要:||
The study was conducted to delineate fundamental mechanisms that initiate the deleterious effect of fuel overloading on reproductive efficacy of broiler breeder hens. Sixty hens at age 26 wk were fed recommended amounts of feed (160 g/d per hen) or allowed voluntary feeding (approximately 30% more than restriction). At age 35 and 50 wk, hens were sampled for further analyzes. Voluntary feeding resulted in poor egg production, high rate of mortality, and abnormal ovarian structure (mainly overt hierarchical follicle atresia at age 35 wk and ovarian involution at age 50 wk). In contrast to feed-restricted hens, voluntary feeding also induced metabolic dysregulations that comprised enhanced adiposity; hepatic triacylglycerol accumulation; and elevated concentrations of plasma glucose, NEFAs, very low density lipoprotein, triacylglycerol, phospholipids, and sphingomyelin (P < 0.05). Furthermore, hepatic and circulating ceramide and sphingomyelin accumulation, and up-regulation of proinflammatory IL-1 beta expression in liver and adipose tissues (P < 0.05) systemically manifested the development of lipotoxicity in feed-satiated hens. Lipotoxicity leading to impaired ovarian dysfunctions, including follicle atresia, ovarian regression, and a decline of circulating estradiol levels (P < 0.05) in feed-satiated hens, was further exemplified by ceramide accumulation and up-regulation of IL-1 beta, serine palmitoyltransferase, and sphingomyelinase transcript abundance, but suppressed protein kinase Akt activation (P < 0.1 to 0.05) within the hierarchical follicles. This study provides the first in vivo evidence of the actions of ceramide and IL-1 beta in mediating overfeeding-induced follicle atresia and progression of ovarian involution in broiler hens. (C) 2012 Elsevier Inc. All rights reserved.
|Appears in Collections:||期刊論文|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.