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標題: Simultaneous determination of components released from dental composite resins in human saliva by liquid chromatography/multiple-stage ion trap mass spectrometry
作者: Hsu, W.Y.
Wang, V.S.
Lai, C.C.
Tsai, F.J.
關鍵字: Biodegradation;Dental composite resin;LC-MS;MS;human gingival fibroblasts;restorative materials;bis-gma;biodegradation;monomers;fertility;electrophoresis;quantification;esterase;tegdma
Project: Electrophoresis
期刊/報告no:: Electrophoresis, Volume 33, Issue 4, Page(s) 719-725.
Dental composite resins are widely used for fixing teeth; however, the monomers used in dental composite resins have been found to be cytotoxic and genotoxic, namely triethylene glycol dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA), and bisphenol A glycol dimethacrylate (Bis-GMA). In this study, we incubated dental composite resins with human saliva for demonstrating the released monomers and biodegradation products. A simple saliva sample dilution method without purification or derivatization was used for quantification. We found that liquid chromatography coupled with multiple-stage ion trap mass spectrometry (LC-MSn) operated in selected reaction monitoring (SRM) mode was able to separate the three monomers within 10 min. The calibration curves were linear (R2>0.996) over a wide range for each monomer in saliva: TEGDMA, 5500 ppb; UDMA, 5100 ppb, and Bis-GMA, 5700 ppb. Furthermore, several biodegradation products were discovered with data-dependent MS/MS scan techniques. Although TEGMA degradation products have previously been reported, we identified two previously unknown UDMA degradation products. The LC-MS/MS method developed in this study was able to successfully quantify monomers and their principal biodegradation products from dental composite resins in human saliva.
ISSN: 0173-0835
DOI: 10.1002/elps.201100571
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