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|標題:||Recovery of active N-acetyl-D-glucosamine 2-epimerase from inclusion bodies by solubilization with non-denaturing buffers||作者:||Lu, S.C.
|關鍵字:||Epimerase;Inclusion bodies;Refolding;Solubilization;d-neuraminic acid;acetylneuraminic acid;sialic-acid;protein;aggregation;circular-dichroism;egg-yolk;renaturation;membrane;bacteria;aldolase||Project:||Enzyme and Microbial Technology||期刊/報告no：:||Enzyme and Microbial Technology, Volume 50, Issue 1, Page(s) 65-70.||摘要:||
Overexpression of recombinant N-acetyl-D-glucosamine 2-epimerase, one of the key enzymes for the synthesis of N-acetylneuraminic acid, in E. coil led to the formation of protein inclusion bodies. In this study we report the recovery of active epimerase from inclusion bodies by direct solubilization with Tris buffer. At pH 7.0, 25% of the inclusion bodies were solubilized with Tris buffer. The specific activity of the solubilized proteins, 2.08 +/- 0.02 U/mg, was similar to that of the native protein, 2.13 +/- 0.01 U/mg. The result of circular dichroism spectroscopy analysis indicated that the structure of the solubilized epimerase obtained with pH 7.0 Iris buffer was similar to that of the native epimerase purified from the clarified cell lysate. As expected, the extent of deviation in CD spectra increased with buffer pH. The total enzyme activity recovered by solubilization from inclusion bodies, 170.41 +/- 10.06 U/l, was more than 2.5 times higher than that from the clarified cell lysate, 67.32 +/- 5.53 U/l. The results reported in this study confirm the hypothesis that the aggregation of proteins into inclusion bodies is reversible and suggest that direct solubilization with non-denaturing buffers is a promising approach for the recovery of active proteins from inclusion bodies, especially for aggregation-prone multisubunit proteins. (C) 2011 Elsevier Inc. All rights reserved.
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