Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68857
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dc.contributor.authorLan, Y.H.en_US
dc.contributor.authorChiang, J.H.en_US
dc.contributor.authorHuang, W.W.en_US
dc.contributor.authorLu, C.C.en_US
dc.contributor.authorChung, J.G.en_US
dc.contributor.authorWu, T.S.en_US
dc.contributor.authorJhan, J.H.en_US
dc.contributor.authorLin, K.L.en_US
dc.contributor.authorPai, S.J.en_US
dc.contributor.authorChiu, Y.J.en_US
dc.contributor.authorTsuzuki, M.en_US
dc.contributor.authorYang, J.S.en_US
dc.date2012zh_TW
dc.date.accessioned2014-06-11T05:57:26Z-
dc.date.available2014-06-11T05:57:26Z-
dc.identifier.issn1741-427Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68857-
dc.description.abstractEmilia sonchifolia (L.) DC (Compositae), an herbaceous plant found in Taiwan and India, is used as folk medicine. The clinical applications include inflammation, rheumatism, cough, cuts fever, dysentery, analgesic, and antibacteria. The activities of Emilia sonchifolia extract (ESE) on colorectal cancer cell death have not been fully investigated. The purpose of this study explored the induction of apoptosis and its molecular mechanisms in ESE-treated HCT 116 human colorectal cancer cells in vitro. The methanolic ESE was characterized, and gamma-humulene was formed as the major constituent (63.86%). ESE induced cell growth inhibition in a concentration-and time-dependent response by MTT assay. Apoptotic cells (DNA fragmentation, an apoptotic catachrestic) were found after ESE treatment by TUNEL assay and DNA gel electrophoresis. Alternatively, ESE stimulated the activities of caspase-3, -8, and -9 and their specific caspase inhibitors protected against ESE-induced cytotoxicity. ESE promoted the mitochondria-dependent and death-receptor-associated protein levels. Also, ESE increased ROS production and upregulated the levels of ATM, p53, and Fas in HCT 116 cells. Strikingly, p53 siRNA reversed ESE-reduced viability involved in p53-mediated ATM/Fas signaling in HCT 116 cells. In summary, our result is the first report suggesting that ESE may be potentially efficacious in the treatment of colorectal cancer.en_US
dc.language.isoen_USzh_TW
dc.relationEvidence-Based Complementary and Alternative Medicineen_US
dc.relation.ispartofseriesEvidence-Based Complementary and Alternative Medicine.en_US
dc.relation.urihttp://dx.doi.org/10.1155/2012/178178en_US
dc.subjectendoplasmic-reticulum stressen_US
dc.subjectu-2 os cellsen_US
dc.subjectdependent pathwaysen_US
dc.subjectin-vitroen_US
dc.subjecttriggers apoptosisen_US
dc.subjectmitotic arresten_US
dc.subjectdna-damageen_US
dc.subjectp53en_US
dc.subjectinductionen_US
dc.subjectdeathen_US
dc.titleActivations of Both Extrinsic and Intrinsic Pathways in HCT 116 Human Colorectal Cancer Cells Contribute to Apoptosis through p53-Mediated ATM/Fas Signaling by Emilia sonchifolia Extract, a Folklore Medicinal Planten_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1155/2012/178178zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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