Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/68911
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dc.contributor.authorNarita, M.en_US
dc.contributor.authorChiba, K.en_US
dc.contributor.authorNishizawa, H.en_US
dc.contributor.authorIshii, H.en_US
dc.contributor.authorHuang, C.C.en_US
dc.contributor.authorKawabata, Z.en_US
dc.contributor.authorSilver, S.en_US
dc.contributor.authorEndo, G.en_US
dc.date2003zh_TW
dc.date.accessioned2014-06-11T05:57:30Z-
dc.date.available2014-06-11T05:57:30Z-
dc.identifier.issn0378-1097zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/68911-
dc.description.abstractThirty mercury-resistant (Hg-R) Bacillus strains were isolated from mercury-polluted sediment of Minamata Bay, Japan. Mercury resistance phenotypes were classified into broad-spectrum (resistant to inorganic Hg2+ and organomercurials) and narrow-spectrum (resistant to inorganic Hg2+ and sensitive to organomercurials) groups. Polymerase chain reaction (PCR) product sizes and the restriction nuclease site maps of mer operon regions from all broad-spectrum Hg-R Bacillus were identical to that of Bacillus megaterium MB1. On the other hand, the PCR products of the targeted merP (extracellular mercury-binding protein gene) and merA (intracellular mercury reductase protein gene) regions from the narrow-spectrum Hg-R Bacillus were generally smaller than those of the R megaterium MB1 mer determinant. Diversity of gene structure configurations was also observed by restriction fragment length polymorphism (RFLP) profiles of the merA PCR products from the narrow-spectrum Hg-R Bacillus. The genetic diversity of narrow-spectrum mer operons was greater than that of broad-spectrum ones. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.en_US
dc.language.isoen_USzh_TW
dc.relationFems Microbiology Lettersen_US
dc.relation.ispartofseriesFems Microbiology Letters, Volume 223, Issue 1, Page(s) 73-82.en_US
dc.relation.urihttp://dx.doi.org/10.1016/s0378-1097(03)00325-2en_US
dc.subjectmercury resistanceen_US
dc.subjectBacillusen_US
dc.subjectmer genesen_US
dc.subjectgenetic diversityen_US
dc.subjectMinamata Bayen_US
dc.subjectgram-positive bacteriaen_US
dc.subjectclass-ii transposonen_US
dc.subjectvolatilizing bacteriaen_US
dc.subjectnatural environmentsen_US
dc.subjectsequence-analysisen_US
dc.subjectsoil bacteriaen_US
dc.subjectmer operonsen_US
dc.subjectgenesen_US
dc.subjectjapanen_US
dc.subjectconservationen_US
dc.titleDiversity of mercury resistance determinants among Bacillus strains isolated from sediment of Minamata Bayen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/s0378-1097(03)00325-2zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextno fulltext-
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