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標題: Establishment of an arabinose-inducible system in Stenotrophomonas maltophilia
作者: Huang, Y.W.
Hu, R.M.
Chiang, Y.T.
Chung, T.C.
Yang, T.C.
關鍵字: range expression vectors;escherichia-coli;tight regulation;promoter;xanthomonas
Project: Folia Microbiologica
期刊/報告no:: Folia Microbiologica, Volume 56, Issue 1, Page(s) 18-22.
A pBBad22T-derived conditioned arabinose (Ara)-inducible expression system was evaluated in Stenotrophomonas maltophilia (an opportunistic pathogen and has gained increasing attention as a cause of healthcare-associated infection). S. maltophilia cannot grow well when Ara is the sole available carbon source. The induction kinetic study, optimal inducer concentration determination, and depletion experiment were performed by using a xylE gene fusion construct, pBxylE, to monitor the expression of pBBad22T in S. maltophilia. For induction survey, the expression of catechol 2,3-dioxygenase (C23O), encoded by xylE gene, continuously increases during an 8-h induced course and can be modulated by different inducer concentrations. The applied induction condition of pBBad22T in S. maltophilia is the inducer concentration ranging from 0.1% to 0.5% for an induction time of 4 h. For repression evaluation, the C23O expression is rapidly turned off within 30 min after the removal of Ara. Accordingly, the established Ara-inducible system can provide a convenient tool for the study of S. maltophilia.
ISSN: 0015-5632
DOI: 10.1007/s12223-011-0008-2
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