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|標題:||Identification and functional characterization of a sesquiterpene synthase gene from Eleutherococcus trifoliatus||作者:||Wen, C.H.
|關鍵字:||Araliceae;Eleutherococcus trifoliatus;sesquiterpene synthase;acanthopanax-trifoliatus;natural-products;vietnamese plants;taiwania-cryptomerioides;bacterial expression;carboxylic-acids;voc;emissions;scots pine;biosynthesis;temperature||Project:||Holzforschung||期刊/報告no：:||Holzforschung, Volume 66, Issue 2, Page(s) 183-189.||摘要:||
In the present study, one sesquiterpene synthase gene in Eleutherococcus trifoliatus was identified and characterized. Full-length cDNA was obtained from stems. It contained an open reading frame of 1671 bp (EtCop) with a predicted molecular mass of 64.5 kDa. The amino acid sequence of EtCop contained the common terpene synthase family motifs RR(x)(8)W, RxR and DDxxD. The recombinant protein from Escherichia coli was incubated with farnesyl diphosphate in order to identify the function of EtCop. The product of EtCop could be identified as an alpha-copaene by means of gas chromatography-mass spectrometry analysis and comparison with an authentic standard.
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