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標題: In vitro micropropagation and ex vitro acclimation of Bupleurum kaoi - An endangered medicinal plant native to Taiwan
作者: Chen, U.C.
Hsia, C.N.
Yeh, M.S.
Agrawal, D.C.
Tsay, H.S.
關鍵字: acclimation;Bupleurum kaoi;container closure;hyperhydricity;proliferation;rooting;scanning electron microscope;tissue-culture;ventilation;propagation;carnation;anatomy;invitro;tobacco;leaf
Project: In Vitro Cellular & Developmental Biology-Plant
期刊/報告no:: In Vitro Cellular & Developmental Biology-Plant, Volume 42, Issue 2, Page(s) 128-133.
This study reports an improved protocol for in vitro-shoot multiplication and ex vitro acclimation of Bupleurum kaoi, an endangered medicinal herb. Nodal segments were cultured in half-strength Murashige and Skoog (MS) basal medium supplemented with different concentrations of benzyladenine (BA) and kinetin. The presence of 0.25 mg l(-1) BA induced the highest number of shoots per explant after 8 wk of culture. Although BA was more effective than kinetin on shoot multiplication, it induced hyperhydric shoots at all concentrations tested. The use of dispense paper (DP) instead of aluminum foil (AF) for container closure was found to reduce hyperhydricity and improve ex vitro acclimation. The best survival rate (61%) was obtained when plantlets were grown in MS basal medium containing 0.5 mg l(-1) indole-3-butyric acid and 0.1-0.2 mg l(-1) alpha-naphthaleneacetic acid using DP as container closure. Leaves of the plant treated with AF6 (two layers of AF as container closure and 6 wk of incubation) lacked epicuticular wax and possessed larger stomata, higher stomata density, and fewer functional stomata compared to those of plants treated with AF2+DP4 (two layers of AF for 2 wk, then replaced AF by three layers of DP for 4 wk) and ex vitro-acclimated plantlets.
ISSN: 1054-5476
DOI: 10.1079/ivp2005744
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