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標題: Apo-8 '-lycopenal Induces Expression of HO-1 and NQO-1 via the ERK/p38-Nrf2-ARE Pathway in Human HepG2 Cells
作者: Yang, C.M.
Huang, S.M.
Liu, C.L.
Hu, M.L.
關鍵字: apo-8 '-lycopenal;HepG2 cells;HO-1;NQO-1;Nrf2-ARE system;antioxidant response element;heme oxygenase-1 gene;chemopreventive;agent sulforaphane;drug-metabolizing-enzymes;transcription factor;nrf2;prostate-cancer cells;factor-kappa-b;oxidative-stress;epithelial-cells;dietary phytochemicals
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 60, Issue 6, Page(s) 1576-1585.
Lycopene and its metabolite apo-10'-lycopenoic acid have been shown to induce phase II detoxifying/antioxidant enzymes through activation of the nuclear factor erythroid-derived 2-like 2 (Nrf2)-antioxidant response element (ARE) transcription system. However, little is known about whether apo-8'-lyocpenal, one of the main metabolites of lycopene in rat livers, in lycopene-containing food, and in human plasma, has similar effects. This study investigated the effect of apo-8'-lycopenal on Nrf2-ARE system mediated heme oxygenase 1 (HO-1) and NAD(P)H:quinine oxidoreductase 1 (NQO-1) expression in human HepG2 cells. It was found that apo-8'-lycopenal (1-10 mu M) significantly increased nuclear Nrf2 accumulation, ARE-luciferase activity, Nrf2-ARE binding activity, chymotrypsin-like activity, and downstream HO-1 and NQO-1 expression, but decreased cytosolic Ketch-like ECH-associated protein 1 (Keap1) expression. Results also revealed that the ERK/p38-Nrf2 pathway is involved in activation of HO-1 and NQO-1 expression by apo-8'-lycopenal using Nrf2 siRNA and ERK/p38 specific inhibitors. In addition, the activation time of lycopene on nuclear Nrf2 accumulation. is slower than that of apo-8'-lycopenal, suggesting that the chemopreventive effects of lycopene may be partially attributed to its metabolites.
ISSN: 0021-8561
DOI: 10.1021/jf204451n
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