Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69315
DC FieldValueLanguage
dc.contributor.authorWang, S.W.en_US
dc.contributor.authorTsai, C.C.en_US
dc.contributor.authorTsen, H.Y.en_US
dc.date1998zh_TW
dc.date.accessioned2014-06-11T05:58:08Z-
dc.date.available2014-06-11T05:58:08Z-
dc.identifier.issn1021-9498zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/69315-
dc.description.abstractThe enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) cells include heat labile (LT) and heat stable toxins (ST), These toxins cause diarrheal in humans and domestic animals. In this study, we combined LT I and ST II gene specific PCR primers into a multiplex PCR system and used this system for the simultaneous detection of LT I and ST II ETEC cells in tap water and environmental waters. Using Chromocult coliform agar (CCA), we found that tap water and underground water were contaminated with 0-10(0) CFU / ml of E. coli and coliform cells while the waters from four rivers near Taichung City were contaminated with 10(2)-10(4) CFU / ml of E. coli and coliform cells. Furthermore, three of the four river waters were contaminated with LT I / ST II and ST II ETEC cells. The high levels of microfloral contamination in these river waters near an urban area should be attended to urgently.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Food and Drug Analysisen_US
dc.relation.ispartofseriesJournal of Food and Drug Analysis, Volume 6, Issue 2, Page(s) 511-516.en_US
dc.subjectmultiplex PCRen_US
dc.subjectLT I and ST II enterotoxigenic E-colien_US
dc.subjectenvironmentalen_US
dc.subjectwatersen_US
dc.subjectpolymerase chain-reactionen_US
dc.subjectenterotoxinen_US
dc.subjectdiarrheaen_US
dc.subjectgenesen_US
dc.subjectpigsen_US
dc.titleUse of a multiplex PCR system for the simultaneous detection of heat labile toxin I and heat stable toxin II Escherichia coli cells in environmental waters near Taichung Cityen_US
dc.typeJournal Articlezh_TW
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
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