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|標題:||Immunologic Characterization and Allergenicity of Recombinant Tyr p 3 Allergen from the Storage Mite Tyrophagus putrescentiae||作者:||Liao, E.C.
|關鍵字:||Basophil histamine release;IgE antibody;Mite group 3 allergens;Storage mite;Trypsin-like serine protease;Tyr p 3 allergen;Tyrophagus;putrescentiae;protease-activated receptor-2;group-iii allergen;dermatophagoides-pteronyssinus;blomia-tropicalis;farinae;cloning;sensitization;reactivity;urban;purification||Project:||International Archives of Allergy and Immunology||期刊/報告no：:||International Archives of Allergy and Immunology, Volume 150, Issue 1, Page(s) 15-24.||摘要:||
Background: The involvement of the storage mite Tyrophagus putrescentiae in allergies has been increasingly reported in many countries. Molecular analysis has shown that group 3 mite allergens are homologous to trypsin. Similar allergens have not been identified in T. putrescentiae. Our aims were to characterize group 3 allergens in T. putrescentiae and to investigate their significance in allergenicity. Methods: cDNAs of PreTyr p 3 and rTyr p 3 from T. putrescentiae were cloned and expressed in Escherichia coli. Native Tyr p 3 (nTyr p 3) was purified from spent growth medium with an affinity column coupling of antibody. Biological activities of rTyr p 3 were compared with nTyr p 3 in terms of IgE activity, enzymatic activity and histamine release. Results: Full-length cDNA of PreTyr p 3 encodes a 285-amino acid trypsin-like protease and acquires enzymatic activity after removing the pre- and pro-sequences. rTyr p 3 is a 26-kDa protein with equivalent IgE reactivity but weaker enzymatic activity than that of nTyr p 3. A limited level of cross-reactivity has been found between rTyr p 3, Der p 3 and Blomia. Eight of 10 T. putrescentiae-sensitized individuals showed > 50% histamine release after triggering with rTyr p 3. Conclusions: Our studies demonstrate that Tyr p 3 is a frequent allergen (58%) in T. putrescentiae-sensitized patients. Since rTyr p 3 displays equivalent biological activities as nTyr p 3, the role of group 3 allergens can be studied using rTyr p 3 to elucidate the pathogenic effects and diagnostic applications of Tyr p 3. Copyright (C) 2009 S. Karger AG, Basel
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