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標題: Display of Fibrobacter succinogenes beta-Glucanase on the Cell Surface of Lactobacillus reuteri
作者: Huang, S.J.
Chen, M.J.
Yueh, P.Y.
Yu, B.
Zhao, X.
Liu, J.R.
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 59, Issue 5, Page(s) 1744-1751.
The aim of this study was to display a rumen bacterial beta-glucanase on the cell surface of a probiotic Lactobacillus reuteri strain. The beta-glucan degrading ability and the adhesion capability of the genetically modified strain were evaluated. The beta-glucanase (Glu) from Fibrobacter succinogenes was fused to the C-terminus of collagen-binding protein (Cnb) from L. reuteri and then expressed by L. reuteri Pg4 as a recombinant Cnb-Glu-His6 fusion protein. Confocal immunofluorescence microscopy and flow cytometric analysis of the transformed strain L. reuteri pNZ-cnb/glu demonstrated that Cnb-Glu-His(6) fusion protein was displayed on As cell surface. In addition, L. reuteri pNZ-cnb/glu acquired the capacity to break down barley beta-glucan and showed higher adhesion capability, in comparison with the parental strain L. reuteri Pg4. To the best of the authors' knowledge, this is the first report of successful display of fibrolytic enzymes on the cell surface of intestinal lactobacilli.
ISSN: 0021-8561
DOI: 10.1021/jf104266x
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